Establishment and evaluation of Pseudomonas aeruginosa-induced sepsis model in mice

doi:10.3969/j.issn.1006-7795.2025.02.019

Abstract

Abstract: Objective To establish a reliable Pseudomonas aeruginosa (PA)-induced sepsis model, providing an effective experimental method for investigating the pathogenicity, antibiotic resistance mechanism and infection-related inflammatory pathways of PA. Methods PA ATCC 27853 was selected as experimental strain. Different concentrations of bacterial suspension were applied to the surface of erector spinae muscle in mice. Echocardiography was performed 24 h after infection to examine cardiac function. Heart, lung, kidney tissue and blood samples were collected. Serum creatinine (Cr), blood urea nitrogen (BUN), inflammatory factors, and cardiac troponin Ⅰ (CTNI) were detected. The pathological changes in the heart, lung, and kidney tissues were observed. Results The survival rates of the 10⁷ CFU/mL group, 10⁸ CFU/mL, 10⁹ CFU/mL, and 10¹⁰ CFU/mL groups were 100%, 93.3%, 73.3%, and 26.7%, respectively. The concentration of interleukin-6 (IL-6) in the PA-infected group was significantly higher than that in the non-infected group. The concentration of tumor necrosis factor-α (TNF-α) in 10⁸ CFU/mL group and 10⁹ CFU/mL group were significantly higher than that in the non-infected group. The left ventricular ejection fraction (LVEF) of 10⁸ CFU/mL and 10⁹ CFU/mL groups decreased significantly compared to the non-infected group, and the CTNI level increased significantly in infected group compared to the non-infected group. Compared with the non-infected group, only the 10⁹ CFU/mL group showed significant statistical differences in Cr and BUN levels, while no significant differences were observed in the other PA-infected groups. The results of histopathology showed that the heart, lung, and kidney tissues of mice in the 10⁹ CFU/mL group were significantly infiltrated by inflammatory cells, with obvious edema of tissue cells, disordered structural arrangement, thickening of alveolar septa, and renal interstitial stenosis. Conclusion The experiment successfully established a sepsis model induced by PA with a bacterial concentration of 10⁹ CFU/mL, which is stable and reliable, and can provide a model basis for exploring sepsis and PA infection diseases.

Key words: Pseudomonas aeruginosa, sepsis, model, infection, mice

CLC Number:

R459.7

Liu Wei, Wang Jingyi, Zheng Xi, Han Yue, Li Wenxiong, Zhang Jin. Establishment and evaluation of Pseudomonas aeruginosa-induced sepsis model in mice[J]. Journal of Capital Medical University, 2025, 46(2): 324-332.

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