Abstract: 65% hepatectomy was performe don 200 rats divided into 3 groups. A preliminary obliteration of the porta hepatis was applied in two groups in different time (30 and 20 mi-nutes respectively),and the other group was used as a control.The regeneration of the liver and survi-val rate were observed in each group. The survival rate was signifi-cantly low in the two groups with preliminary obliteration of the porta hepatis (P<0.01) but not affected by the duration of obliteration(P> 0.05)The hepatic regeneration was almost the same among the 3groups.