Journal of Capital Medical University ›› 1990, Vol. 11 ›› Issue (4): 279-282.

• 多肽研究 • Previous Articles     Next Articles

Biolgical Activity of a New AngiotensinⅠConverting Enzyme Inhibitor and a N-terminal Fragment of Substance P

Guo Chunyuan1, Liu Chuandi1, Sheng Shuli2, Liu Aimin2, Chen Yanjiang2, Hu xiaoyu3   

  • Received:1900-01-01 Revised:1900-01-01 Online:1990-10-15 Published:1990-10-15

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Abstract: A new angiotensin I converting enzyme inhibitor(AI, Pro-Thr-His-Ile-Lys Trp-Gly-Asp) and a N-terminal fragment of substance P (SP3-4, Gly-Phy) were synthesized by solid phase method. Their capacity to inhibit angiotensin I converting enzyme (ACE) seperated from human blood, brain and lung was tested by biochemical assay. Results showed that 1. All of the synthetic peptides were_assayed and their purity was>95% as established by HPLC analysis. 2. The AI inhibited the ACE from human blood(IC50 19±4.6μmol/L) brain (IC50 26±6.3μmol/L) and lung (2 cases, IC5011.9～14.1μmol/L) but the SP3-4 failed to inhibited the ACE from human being.

Key words: dangiotensin I converting enzyme inhinsin biotor, N-terminal fragment of substance P, anigiotensin converting enzyme