New Culture Method for Primordial Germ Cell——Application of Sert oli Cells

Journal of Capital Medical University ›› 2004, Vol. 25 ›› Issue (1): 15-18.

• 论著·基础研究 • Previous Articles Next Articles

New Culture Method for Primordial Germ Cell——Application of Sert oli Cells

Lu Xin, Xu Qing, Liang Yuanjing, Shi Xiaolin

Department of Reproductive Medical Center, Capital University of Medica l Sciences

Received:2003-01-04 Revised:1900-01-01 Online:2004-01-15 Published:2004-01-15

Abstract

Abstract: The aim was to investigate the effects of sertoli cells (SCs) on the growth an d proliferatio n of primordial germ cells (PGCs). Primordial germ cells were co-cultured with homologue fibroblasts from genital ridge or with sertoli cells. Result: When co -cultured with SCs, PGCs colonies and times of passage were greater than those co-cultured with homologue fibroblasts. Conclusion: Sertoli cells can enhance p rimordial germ cells' proliferation in cell culture.

Key words: sertoli cells, cell culture, primor dial germ cells

CLC Number:

R321.1

Lu Xin;Xu Qing;Liang Yuanjing;Shi Xiaolin . New Culture Method for Primordial Germ Cell——Application of Sert oli Cells[J]. Journal of Capital Medical University, 2004, 25(1): 15-18.

[1]	ZHAI Meng-yao;GAO Zhi-qiang;LIANG Ying-juan;SHEN Peng. Experimental Study on Methods for Primary Culture of Geniculate Ganglion Cells and Spiral Ganglion Cells of Adult Guinea Pigs [J]. Journal of Capital Medical University, 2009, 30(6): 760-765.
[2]	Zhu Wanwan;Wang Shuyan;Wu Di;Xu Yanling;Fu Linlin;Guan Yunqian;Zhang Yu. Comparison between Trypsinization and Collagenase Treatment in Passaging of Human Embryonic Stem Cells [J]. Journal of Capital Medical University, 2008, 29(4): 445-450.
[3]	Xing Hu;Fang Jugao;Yu Zhenkun;Ma Lijing;Wang Hong;Fan Erzhong;Chen Xiaohong;Han Demin. A Study on the Effect Factors of the Primary Culture of Laryngeal Squamous Cell Carcinoma [J]. Journal of Capital Medical University, 2005, 26(3): 242-245.
[4]	Zhao Wenming;Zheng Qun;Liu Zhenlong;Ping Guoling;Ding Lixin;Shi Weixian;Liu Hailin. Effect of Chinese Herb-catechu Extract on Influenza A Virus [J]. Journal of Capital Medical University, 2005, 26(2): 167-170.
[5]	Shangguan Fangfang;Shi Xiaolin. Trophic Effect of Sertoli Cells on Cerebral Cortex Neurons in Vitro [J]. Journal of Capital Medical University, 2003, 24(3): 247-250.
[6]	Feng Mingli;Yong Yimin;Shen Huiliang;Hu Huaijian. Effect of bFGF Stimulating Proteoglycan Synthesis on Rabbit Articular Chondrocyte Culture in Vitro [J]. Journal of Capital Medical University, 2003, 24(3): 317-320.
[7]	Wang Hongyun;An Yihua;Wan Hong;Liu Kui;Zhang Yazhuo;Wang Zhongcheng . Comparision of Culture Methods in Vitro of Rat Embryonic Neural Stem Cells [J]. Journal of Capital Medical University, 2002, 23(4): 316-318.
[8]	Feng Mingli;Yong Yimin;Shen Huiliang;Hu Huaijian . Proliferation Effects of bFGF on Rabbit Articular Chondrocyte Culture in Vitro [J]. Journal of Capital Medical University, 2001, 22(3): 244-247.
[9]	Zhang Hua;Sun Haime;Guo Chongjie;Gao Fuyun;Zhao Tiande;Wei Yulin. Changes of the Ultrastructure of Human Hepatocellular Carcinoma Cells and Fibroblasts Cocultured in Vitro [J]. Journal of Capital Medical University, 2001, 22(1): 10-12.
[10]	Feng Mingli;Yong Yimin;Shen Huiliang;Hu Huaijian . Effects of bFGF on Collagen Synthesis and Stabilization of Phenotypes of Cultured Rabbit Articular Chondrocyte in Vitro [J]. Journal of Capital Medical University, 2001, 22(1): 55-58.
[11]	Shi Xiaolin;Xu Qing;Li Weihong;Zhu Dingshou . A Study of Follicular Cells of Adult and Embryonal Rats in Vitro [J]. Journal of Capital Medical University, 1998, 19(3): 201-204.
[12]	Dong Cangzhuan;He Fang. Effects of Glycine on the Wandering Capicity of Wounded Epidermal Cells [J]. Journal of Capital Medical University, 1993, 14(1): 5-7.
[13]	Zhang Guofeng;Zhao Chunli;Lu Qiang;Liou Yujun. The in Vitro Culture of Adult Human and Rat's Chromaffin Cell of Adrenal Medullary Tissue [J]. Journal of Capital Medical University, 1990, 11(3): 180-185.
[14]	Yan Lin;Pan Lizhen;Chen Peihui;Zhang Yi. The Establishment and Characterization of a Cell Line (CT-188) from Larvae of Culex Tritaeniorhynchus [J]. Journal of Capital Medical University, 1989, 10(4): 289-293.

New Culture Method for Primordial Germ Cell——Application of Sert oli Cells

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 14

Recommended Articles

Metrics

Comments