Abstract: The study was designed to observe the inhibited effect of the antisense oligonuc leotide(ASON) targeting IGF-I on proliferation,differatiation and apoptosis of the liver cancer cell line BEL-7402 by antisense oligonucleotid e transfection. Antisense oligonucleotide against the insulin like growth factor I was synthesized and transfected to human liver cancer cell line B EL-7402 cells by Lipofectin kits. MTT method was used to measure the cell proli feration. The expression level of CEA and AFP in the supernatant of transfect ce ll culture was measured by RIA. The apoptosis of BEL-7402 cells was observed by TUNEL techniques. It was shown that the lipofectin ASON significantly inhibite d the growth of BEL-7402 cells. The expression of AFP and CEA in the supernatan t of transfected cell culture were significantly lower as compared to the parent cells (P<0.05 and P<0.01 respectively). Antisense IGF-I oligonucle otide transfected to human liver cancer cell line BEL-7402 can lead to a reduct ion of proliferation and differatiation. It can also induce the apoptosis of cells .

Key words: insulin like growth factor I, transfec tion, antisense, oligonucleotides, liver cancer cell