Abstract:

The aim was to explore the isolation, purification and differentiation of umbil ical cord blood cells into neuronlick cells in vitro. Human cord blood samples were obtained, cultured and induced with fectors. Identification was performed with immunochemistry method. Umbilical cord blood could proliferate an d generate clones when cultured in vitro. These cell grew rapidly and different iated into neuronlike cells and astrocytelike cells. The cell clones proliferate d from stem cell could express nestin antigen and the differentiated cells expre ssing GFAP or NSE antigen respectively. In this study, bFGF and EGF in fluencing the induction and differentiation of Umbilical cord blood were observed. It suggests that umbilical cord blood cells have selfrenewal and differentiat ion abilities. They might differentiate into neuronlike and astrocytelike cells which express GFAP or NSE. The umbilical cord blood can be considered the ideal seed cells of the neural stem cells.

Key words: umbilical cord blood, induction diff erentiation, neuronlike cell