Abstract:

Objective To examine the minimum effective dosage of irradiation which can kill the cervical cancer cells in the blood. Methods Human cervical cancer cell line-HeLa cells were exposed to radiation in different dosage. The inhibitory rate of cell growth and colony were tested. HeLa cells were mixed with human red blood cells, and exposed to radiation. Then, these two kinds of cells were divided by density gradient centrifugation, and the rate of colony inhibition were tested. The blood samples were collected from 40 patients with different female malignancies, and 2,3-DPG-one of the functional indications of carrying oxygen by red blood cells, was tested after being exposed to irradiation or not. Results Either the cell growth inhibitory rate or the colony inhibitory rate of the HeLa cells was 100%(P<0.01) after being exposed to 12 Gy irradiation, meanwhile, the value of 2,3-DPG was nearly unchanged(P>0.05). Conclusion 12 Gy irradiation can kill the cervical cancer cells in the blood efficiently, and have no affection to carrying oxygen of red blood cells. The results above metioned provide the experimental data of intraoperative autotransfusion for the clinical trial in future.

Key words: cervical cancer, HeLa cell line, autotransfusion, blood irradiation