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Table of Content

    24 April 2005, Volume 26 Issue 2
    Recent Advances in Clinical Epidemiologic Study of Cardiovascular Disease in China
    Hu Dayi;Yang Jingang;Tong Qiguang
    2005, 26(2):  99-102. 
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    Multiple clinical epidemiologic study of cardiovascular disease in China had been conducted, especially in atrial fibrillation, heart failure, hypertension and hyperlipidemia in the past 5 years. Through the results of these studies, we gained insight into detailed information on the distribution and characteristics of the cardiovascular disease in Chinese population, and acquired objective assessment to improve the prevention and treatment of cardiovascular disease in China.

    Experimental Research on Construction of 3-Dimen-sional Scaffolds with Absorbable Collagen-Chitosan
    Zhang Jian;Chen Bing
    2005, 26(2):  103-107. 
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    Objective To investigate the feasibility for fabricating the 3-dimensional scaffolds with collagen-chitosan applied to tissue engineering of blood vessel. Methods Different proportion of collagen to chitosan suspensions were used as the biomaterials to construct 3-Dscaffolds in vitro. And the 3-Dstructure in the scaffolds were observed. The scaffolds biodegradability and biocompatible property were observed by imbedding them subcutaneously or dipping in the solution of collagenase (Ⅰ), bacteriolysin, trypsinase solutions. Results The fabricated scaffolds had the relatively uniform structure, with perfect biocompatibility. The different proportions for collagen to chitosan could work on the construction character in scaffolds, the chitosan could influence collagenase degradation for scaffolds and prolong the degradation progress in vivo. Conclusion The 3-dimentional scaffolds made from collagen-chitosan, with relatively uniform and excellent biocompatibility, can be used in vascular tissue engineering research.

    Surgical Repair of Pulmonary Atresia with Intact Ventricular Septum
    Li Xiaofeng;Li Zhongzhi
    2005, 26(2):  108-110. 
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    Objective To investigate a more effective surgical approach to treat pulmonary atresia with intact ventricular septum (PA-IVS). Methods From January 2001 to June 2004, 11 patients with PA-IVSwere enrolled in a retrospective study, aged from 34 d to 32 month old, averaged (8.00±9.04) months, with body weight from 4.2 kg to 10 kg, (6.67±1.86) kg. All of 11 patients complicated with PDA and ASD/PFO, 10 patients with RVOTO, 2 patients with right ventricle or tricaspid valve hypoplasia. One patient received pulmonary valvotomy without CPB, and eight patients had transventricular pulmonary valvotomy with patch repaired, one patient received operation for Glenn shunt, and the other one had Fontan procedure. Results The early post-operation mortality was 18.1% (2 cases), and the 2 cases were complicated with severe right ventricular, one case had hypoplasia pulmonary valvotomy and enlarged right ventricle with Gore-tex patch, the another one had Fontan procedure. Eight patients were followed-up for 1 month to 3 years; the blood O2 saturation of eight patients was over 0.9. Two cases remained in mild stenosis of the pulmonary valve or in the right ventricular outflow tract. Conclusion PA-IVS patients should be surgically repaired as early as possible and the surgical results depends on the growth of right ventricle and tricuspid valve.

    Experimental Research on the Change of Gene Expression of Interleukin-1β, Interleukin-6 and Tumor Necrosis Factor-α in Right Atrial ppendages of Patients with Chronical Atrial Fibrillation
    Wang Changhua;Hu Dayi;Tang Chuzhong;Wu Mingying;Mei Yunqing
    2005, 26(2):  111-115. 
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    Objective To investigate whether the gene expression of interleukin-1β(IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α(TNF-α) in right atrial appendage is altered in patients with chronic atrial fibrillation. Methods 48 patients with rheumatic heart disease were enrolled. Twenty-seven patients had no history of atrial fibrillation, and twenty-one patients had atrial fibrillation. Atrial tissue was obtained from the right atrial appendage during open heart surgery. The mRNAexpression of IL-1β, IL-6 and TNF-α was measured by reverse transcription-polymerase chain reaction (RT-PCR). The protein expression of IL-1β and TNF-α was detected by immunhistochemical method. The fibrosis of right atrial appendage was detected by Masson staining. Results The expression of IL-1β/GAPDH, IL-6/GAPDHand TNF-α/GAPDHwas significantly increased in patients with chronic atrial fibrillation compared with patients with sinus rhythm (1.48±0.38 vs 0.78±0.27, P<0.001; 1.46±0.48 vs 0.73±0.25, P<0.001; 1.71±0.98 vs 0.73±0.29, P< 0.001). The fibrosis of right atrial appendage was significantly increased in patients with chronic atrial fibrillation. The protein expression of IL-1β and TNF-α was significantly increased in patients with chronic atrial fibrillation. Conclusion The mRNA expression of IL-1β, IL-6 and TNF-α is significantly increased in patients with chronic atrial fibrillation, inflammation may be one of mechanisms for the development and maintainance of atrial fibrillation.

    Effects of Atorvastain on Reactive Oxygen Species, Expression of p22phox in Aorta and Level of Superoxide Dismutase of SHR
    Li Weiping;Sun Ming;Jia Sanqing
    2005, 26(2):  116-119. 
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    Objective The study was to investigate the alterations and the correlations among reactive oxygen species(ROS), p22phox mRNA expression, and activity of superoxide dismutase(SOD) in aorta of SHRand effects of atorvastain. Methods Wistar-Kyoto(WKY) rats were used as control, SHR were treated with atorvastain 〔50 mg/(kg·d)〕 for 30 days. Blood pressure, levels of serum SOD, nitric oxide(NO), ROS and p22phox mRNA expression in aorta were measured. Results After 30 days' treatment with atorvastain, blood pressure, levels of vascular ROS, and mRNA expression of p22phox in aorta decreased and the levels of serum SOD and NOincreased significantly. Aortic ROS was positively correlated with p22phox mRNA expression and negatively correlated with SOD. Conclusion The increased genesis of aortic ROS in SHRis caused by upregulation of p22phox mRNA expression and reduction of SOD. Atorvastain can reduce ROSby down regulating p22phox expression and increasing SOD.

    Effect of Nonhypertension Lowing Dosage of Spironolactone on Renal Arterioles Remodelling in Nω-nitro-L-arginine-methyl ester/salt Induced Hypertensive Rats
    Wan Xinhong;Liu Xiaohui;Li Yanhui;Yang Xinchun;Zhang Lin
    2005, 26(2):  120-123. 
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    Objective To evaluate whether the nonhypertension lowing dosage of spironolactone, was able to prevent the renal arterioles remoderlling in Nω-nitro-L-arginine-methyl ester(L-NAME) and salt induced hypertensive rat. Methods Twenty-two male SDrats were divided into 3 groups randomly: group received tap water (C) as control, group 2 received L-NAME (L), group 3 received L-NAME plus spironolactone by gastric gavage (S). All the rats were fed with 1% salt water except the rats in group C. Eight weeks later biochemical assessment of serum were evaluated. The vessel chamber diameter (CD)and area(CA), the vessel middle layer thickness(MT) and area(MA) and the ratio of middle layer thickness to chamber diameter(M/C) were calculated by microscopy observation, the ratio of middle layer fiber area to middle layer area was determined. Results The blood pressure in group Land Sshowed an expected hypertension compared with those in group C(P<0.01), and there is no significant different between group Land S(P>0.05). Significant differences were found in the CD, MT, CA, M/Cand the fibrosis in the middle layer of renal arterioles between Land C, S. Conclusion The nonhypertension lowing dosage of spironolactone can prevent the renal arterioles remoldelling effectively.

    Prevalence of Hyperuricemia in Patients with Cardiovascular Risk Factors
    Zhang Lijing;Hu Dayi;Yang Jingang;Xu Yuyun;Li Tianchang;Wang Guohong;Shi Xubo;Lu Yafeng
    2005, 26(2):  124-127. 
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    Objective The purpose of the present study was to investigate the prevalence of hyperuricemia in people with cardiovascular risk factors and the relationship between serum uric acid levels and the various cardiovascular risk factors. Methods This study was performed through cross-sectional survey. Patients with cardiovascular risk factors were enrolled from June 2004 to December 2004 consecutively. Serum uric acid was measured and data were analyzed to investigate the prevalence of hyperuricemia both in men and women, and to identify the risk factors. Results 1600 patients were enrolled (821 men and 779 women ranging from 26 to 99 years old). The prevalence of hyperuricemia was 20.58% in men and 30.55% in women. Hyperuricemia was positively correlated with hypertension, hypercholesterolemia, hypertriglyceridemia, high level of serum creatinine, and the administration of diuretics in men, and was positively correlated with age, hypertension, hypercholesterolemia, hypo-HDL cholesterolemia, high level of serum creatinine, and the administration of diuretics in women as well. The proportion of hyperuricemic patients with 3 or more risk factors is higher than that of non-hyperuricemic patients (76.92 vs 59.67% in men; 67.64% vs 46.77% in women). Conclusion The prevalence of hyperuricemia in patients with cardiovascular risk factors is high. Cardiovascular risk factors often cluster in hyperuricemia patients. The prevalence of hyperuricemia in women is higher than that in man. Apart from hypertention, hypercholesterolemia, high level of serum creatinine and the use of diuretics, hyperuricemia are also positively correlated with age and hypo-HDL cholesterolemia in women.

    Clinical Characteristics of Aspirin Resistance in Patients with Cardiovascular Diseases
    Zhu Zhengyan;Hu Dayi;Shi Xubo;Wang Chunbo;Zhu Jingyan;Yang Jingang;Li Tianchang
    2005, 26(2):  128-131. 
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    Objective The study was designed to investigate the clinical characteristics of aspirin resistance in patients with cardiovascular diseases.Methods 326 patients with stable cardiovascular diseases were evaluated for aspirin resistance with idetection of optical platelet aggregation induced by arachidonic acid(AA) and adenosine diphosphate(ADP). Aspirin resistance was defined as an average aggregation of over 20% with 0.5 mmol/L AAand over 70% with 10 μmol/L ADP. Aspirin semiresistance met one, but not both of the above criteria. Results 4.9% of the patients were aspirin resistant and 27.6% were aspirin-semiresistant. The clinical characteristics of aspirin resistant patients compared with aspirin sensitive patients were: 1) most of the aspirin-resistant patients were senile; 2) female patients were more frequent than male; 3) patients with diabetes were dominant in number; 4) it had nothing to do with hypertension, hyperlipodemia, red blood cell count, hemoglobin and platelet count; 5) it was relatively rare in smoking population. Conclusion The incidence of aspirin resistance is similar to other studies, in addition to aging and female gender, diabetes is also a risk factor in aspirin resistance.

    Effects of Pro-UK on Coagulation and Fibrinolysis Compared with UK in Patients with Acute Myocardial Infarction
    Xu Ji;Hua Qi;Liu Lisong;Wan Yungao;Li Dongbao;Xu Zhaoping;Jiang Yan;Yang Xu;Li Tiande;Hu Dayi;Liu Rongkun;Yang Zheng
    2005, 26(2):  132-135. 
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    Objective To observe the effects of Pro-UK on coagulation and fibrinolysis compared with UK in patients with acute myocardial infarction. Methods 114 patients with acute myocardial infarction who accorded with the indications of thrombolytic therapy were randomly divided into four groups (Pro-UK 30 & 40 mg: 26 cases; 50 mg: 29 cases ; 60 mg: 28 cases; UK: 31 cases). Plasminogen(PLG), D- dimer (D-D), tissue-type Plasminogen Activator(t-PA), Plasminogen Activator Inhibitor(PAI) and fibrinogen (Fib) were measured immediately before and 2,24,48 h after thrombolysis initiation. The effect of the thrombolytic therapy and patient's bleeding complications were also observed. Results 2 h after thrombolytic therapy, PLGand PAI of each group were significantly lower(P<0.01), D-Dand t-PAwere significantly higher than that before thrombolytic therapy in each group(P<0.01); Fib of UK group 2 h after therapy became significantly lower than that before therapy(P<0.01), but the changes in Pro-UK groups were not significant, all changes recovered 24 h after therapy; 2 h after thrombolytic therapy, Fib of Pro-UKgroups were significantly higher than that of UKgroup(P<0.01), the results at other times were no significantly different; there were no significant differences between each group in terms of therapeutic effects and bleeding syndrome; 2 h after therapy, PAI of reperfusion group was significantly lower than that of non-reperfusion group(P<0.01), other results were not significantly different. Conclusion There is significant effect on the activation of systemic fibrinolysis in UKgroup, but Pro-UKhas little effect on the systemic fibrinolysis.

    Clinical Value of Adenosine Stress Echocardiography in Diagnosis of Coronary Artery Disease
    Chen Xin;Yang Ming;Zou Fengjun;Ding Wuling;Zhang Xiaohang;Wang Qing;Gao Kang;Pang Guozhong;Yin Zhaoxia;Han Ling;Hu Wenze;Cen Qiang
    2005, 26(2):  136-139. 
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    Objective The aim of the study was to assess the clinical value of adenosine stress echocardiography in diagnosis of CAD. Methods Adenosine stress echocardiography(ASE) was performed in 100suspectable CAD patients with infusion of adenosine 140 μg/(kg·min) in 6min. Left ventricular wall motion was recorded during the procedure. Positive criterion was defined as the appearance of wall motion abnormality(WMA). And then among them 64 patients underwent the coronary angiography a week after ASE. Coronary angiography was taken as the golden standard for CAD and comparion of ASE. Results The total sensitivity of this ASE was 68.8% and the total specificity was 81.2% in diagnosis of CAD. Furthermore the sensitivity for detection of myocardial ischemia in one, two and three-vessel CAD sub-groups were 60.6%, 70.0% and 83.3% respectively. Side effects due to adenosine in fusion were always minimal and well tolerated by the patients. Conclusion Adenosine stress echocardiography can be safely performed in suspectable CAD patients with high sensitivity and specificity.

    Prevalence and Risk Factors of Anxiety and Depression in Hypertensive Patients
    Zhang Fan;Hu Dayi;Yang Jingang;Xu Yuyun;Li Tianchang;Shi Xubo
    2005, 26(2):  140-142. 
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    Objective To investigate prevalence and risk factors of anxiety and depression in hypertensive patients.Methods The investigation was based on interview with consecutive hypertensive patients selected at random in cardiovascular outpatient clinics from nine hospitals in Beijing (patients with language or movement barriers due to cerebrovascular disease were excluded). The patients were required to fill in Self-Rating Anxiety Scale and Self-Rating Depression Scale. Meanwhile other basic information was also collected.Results Atotal of 2274 hypertensive patients were enrolled, with 1025 males(45.1%) and 1249 females(54.9%). The prevalence of anxiety was 38.5% and that of depression was 5.7%. The independent risk factors in hypertensive patients for anxiety were: female, juvenility, single, better educational background, blue-collar, lower income, longer course of diseases, higher grade of hypertension and with coronary artery disease. Risk factors in hypertensive patients with depression were: advanced age, single, female, longer course of diseases, lower income, lower education degree, complicated with coronary artery disease, and with diabetes mellitus.Conclusion The prevalence of anxiety in hypertensive patients is significantly higher than that of depression. Female,single, longer course of diseases, lower income, higher grade of hypertension and coronary artery disease are common indepentent risk factors in anxiety and depression. In addition, juvenility, blue-collar patients, better educational background are prone to anxiety; elderly patients with poor educational background are prone to depression.

    Epidemiology of Cardiovascular Disease and Risk Factors in Chinese Population
    Wang Wei;Zhao Dong
    2005, 26(2):  143-146. 
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    Cardiovascular diseases (CVD) have become the leading contributor to death in China for the recent 20 years. Alarge number of epidemiologic studies of CVD were carried out in different regions of China. The study summarized the epidemiologic characteristics of CVD risk factors, distribution and the general tendency. The main results were as follows: Beijing area was different from western countries, the characteristics of CVD in Chinese population showed that both the incidence and mortality rate of coronary heart disease were low, and those of cerebrovascular disease were high. There were significant geographic variations in both CVDincidence and mortality rate, with higher rates in north and lower rates in south. In the past several decades, the increasing trend of CVD incidence was observed in Chinese population, while the CVD related mortality dereaded in most developed countries. Althrough total serum cholesterol (TC) and body-mass index (BMI) of Chinese population were lower than those of western countries, the current trends were increased significantly. Smoking rates in Chinese men ranked No. 1 in the WHO-WONICA study. The CVD epidemic in China was attributable to increased trends of main risk factors of cardiovascular diseases. Comprehensive prevention and intervention for main cardiovascular risk factors could reduce the risk of CVD.

    Interpretation of 2004 China Guidelines on Prevention and Management of High Blood Pressure
    Hua Qi
    2005, 26(2):  147-150. 
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    In 2004China guidelines, normal blood pressure is defined as less than 16.0/10.6 kPa(120/80 mmHg). Cardiovascular risk factors and target organ damages in patients with hypertension are determined by more indexes, such as low density lipoprotein cholesterol, high density lipoprotein cholesterol, intra-abdominal obesity (waist circumference), Creactive protein, microalbuminuria and increase of carotid intima-media thickness (IMT≥0.9 mm). In 2004 China guidelines, goal blood pressure is less than 18.6/12.0 kPa(140/90 mmHg), and in patients with diabetes mellitus or nephropathy it is 17.3/10.6 kPa(130/80 mmHg). In elderly patients with isolated systolic hypertension, the goal blood pressure is 20.0/12.0 kPa(150/90 mmHg) or lower.

    How to Correctly Select the Indications and Procedures of Operational and Interventional Therapy for Arterial Occlusion in Different Sites of Lower Extremity
    Wu Qinghua
    2005, 26(2):  154-155. 
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    It is extremely important to correctly select the indications and procedures of operational and interventional therapy for arterial occlusion in different sites of lower extremity in order to secure the patients' affected limbs and improve their life quality. This article reviewed the main occlusive sites of arteries in lower extremity and correct selections of operations and procedures, ie: for occlusion of abdominal aorta or/and arteria iliaca; occlusion of superficial femoral artery and occlusion of popliteal artery or/and intra-popliteal artery.

    Synthesis and Simulation of PAMAM Dendrimer
    Tang Jingcheng;Ye Ling;Peng Yaguang;Zhang Liangren
    2005, 26(2):  156-158. 
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    Objective The 3-Dimensional structure of G3, G4, and G5 PAMAM dendrimers were investigated by molecular dynamic simulation. Methods Simulation was performed on SGI Octane2 workstation. Specifically, Insight II 2000 software was employed to simulate the structure of PAMAM dendrimers under vacuum condition. Results With the increasing of the generation (G3~G5), the Dendrimers undergoes a transition from an opener, asymmetrical structure to a more compact, symmetrical global structure. Thus, for G5 PAMAM dendrimer, it has large amount of external amine groups and internal cavities as well. Conclusion The computational simulation data show that G5 PAMAM is a highly ordered, 3-demensional global macromolecule. The internal cavities and external functional groups offer G5 PAMAM dendrimer a great potential as carrier for various drugs.

    Construction of Eukaryotic dMAR Expression Vector and its Retardance on EGFP Expression
    Sun Licui;Qi Yahui;Zhang Jingyi;Yan Yudong;Yin Hong
    2005, 26(2):  159-162. 
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    Objective To study the regulation role of dMAR on EGFPexpression. Methods The dMAR eukaryotic expression vectors were constructed by inserting 850 bp dMAR fragment into pEGFP-C1 vector at the downstream of EGFP with two different orientations. pEGFP/dMAR(+)(sense) and pEGFP/dMAR(-)(antisense) were made. The regulation of EGFP by overexpression dMAR was assessed with fluorescent microscope and flow cytometry(FCM) after the pEGFP/dMAR(+) or pEGFP/dMAR (-) vector transfected into COS7 cells. Results The EGFPexpression of pEGFP-C1, pEGFP/dMAR(+) and pEGFP/dMAR(-) was 45.1%、40.5% and 11.3% respectively 48 h post-transfection. Conclusion The expression of EGFPwas significantly inhibited by dMAR(-) co-expression. In contrast, the expression of EGFP was not affected by dMAR(+) co-expression.

    Changes of Endometrial Vessels in Dysfunctional Uterine Bleeding
    Zhang Guojing;Lu Xin;Xu Qing;Wei Jingbo;Shi Xiaolin
    2005, 26(2):  163-166. 
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    Objective To study the common changes in pathology of dysfunctional uterine bleeding(DUB) caused by endocrine disturbance and birth control medicine (Norplant). Methods Histology and immunohistochemistry were used to demonstrate the expression of CD34 in endothelial cell, laminin in basement membrane in endometrial vessels as well as morphometric analysis of the endometrium of DUB (contraceptive and endocrine disturbance). Results The bleeding caused by birth control medicine(Norplant)was diffuse and the growth of these endometrium was inhibited, while the bleeding caused by endocrine disturbance was localized and the growth of those endometrium appeared different state. The common ground of the two groups was that the blood vessels in bleeding position were not integrative and the expression of CD34 in endothelial cell and LNin basement membrane in blood vessel were distinctly weakened. Conclusion Whatever the inducement of DUBis, the direct changes of DUB is the decreasing of CD34 in endothelial cell and LNin basement membrane of capillary vessel.

    Effect of Chinese Herb-catechu Extract on Influenza A Virus
    Zhao Wenming;Zheng Qun;Liu Zhenlong;Ping Guoling;Ding Lixin;Shi Weixian;Liu Hailin
    2005, 26(2):  167-170. 
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    Objective To investigate the inhibitory effect of catechu extract to influenza Avirus. Methods The inhibitory cytopathic effects (CPE) of catechu extract on influenza Avirus were observed by MDCK cell culture techniques. The inhibiting influenza Avirus effect of catechu extract was observed by means of chick-embryo culture, and the inhibitory activity of catechu extract on influenza Avirus in vitro was observed by the hemagglutination inhibition test. Results 1) Cytotoxicity of catechu extract was lower and the catechu extract could inhibit influenza Avirus from infecting MDCK significantly. 2) The catechu extract showed significant inhibition on the influenza Avirus in the groups within the limits of dose on chick-embryo culture and the hemagglutination inhibition titer was over 8 times higher than the control group. 3) The catechu extract had distinctly inhibitory functions on influenza Avirus, and the hemagglutination inhibition titer was over 16 times higher than the control group. Conclusion The chinese herb-catechu extract has strong effect on anti-influenza Avirus.

    Different Plasma Insulin Levels on Systolic Blood Pressure of Rats
    Zhong Liyong;Fung M L
    2005, 26(2):  171-174. 
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    Objective The purpose of study was to explore the effects of different plasma insulin levels on systolic blood pressure of rat model. Methods Experimental animals were divided into three groups randomly: insulin osmotic mini-pump group (induced-hyperinsulinemia); alloxan-induced diabetes group (induced-hypoinsulinemia & hyperglycemia) and normal control group (normal insulinemia). Hyperinsulinemia animal model was induced by injection of insulin subcutaneously through of a mini-pump, hypoinsulinemia and hyperglycemia animal models were induced by intraperitoneal injection of alloxan (40 mg/kg), and then the plasma insulin and glucose levels were measured on the experimental day of 0, 2, 5, 8, 11, 15, respectively, the systolic blood pressure was measured on the experimental day of 1, 4, 7, 10, 13, 16, respectively. Results The insulin osmotic mini-pump induced significant hyperinsulinemia compared with control group(P<0.05), and the significant systolic blood pressure uprising was observed on the experimental day of 10, untie the end of the experiment in the insulin osmotic mini-pump group.There was a significant difference between hyperisulinemia group and the others(P<0.01), otherwise, there was no obviously systolic blood pressure increasing in both control group and alloxan group, although the systolic blood pressure of alloxan group to be observed an increasing trend on the experimental day of 13 and 16, respectively, but there was no significant statistic meaning compared with control group(P>0.05). Conclusion Exogenous insulin can induce uprising of systolic blood pressure of rats; there is no significant variations of systolic blood pressure in model of hypoinsulinemia and hyperglycemia during the experimental period.

    Histologic Effect of Recombinant Human Growth Hormone on Acute Hepatic Necrosis in Mice
    Xiong Feng;Ning Huijuan;Jin Rui;Xu Jian;Xie Xianchun;Ji Zhonghe;Lang Zhenwei;Duan Zhongping
    2005, 26(2):  175-178. 
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    Objective To investigate the histologic effect of recombinant human growth hormone (rhGH) on acute hepatic necrosis in mouse model which induced by tumor necrotic factor-α (TNF-α)+D-galactose(D-Gal). Methods Mouse model with acute hepatic necrosis was created. Fifty male BALB/C mice were randomly divided into 5 equal groups, which were blank-control group(A), model-control group(B) and experimental groups(C, Dand E), respectively. D-Gal+TNF-α were intraperitoneally administered to every mouse in group B. In experimental groups, D-Gal+TNF-α in the same dosage as that in group Band varied dosage of rhGH (dosage: C<D<E) were intraperitoneally given to every mouse. Every one in group Awere given the same volume of normal saline. Once any one of all mice was found on the brink of death, all mice was killed and a piece of liver tissue of every mouse was taken for light microscopy. At the same time, the pieces of liver tissues from the mice (one selected randomly from every group) were taken for electron microscope observation. Blood samples of every mouse in group Aand Bwere taken for ALTand ASTserum assessment just before the mice's being killed. Results There was significant difference between the values of serum ALTassessment in group Aand those in group B(P= 0.000), and so did the values of serum AST assessment(P=0.000) between in group Aand group B. The hepatic necrosis area percentage in group Bwere significantly different from that in group E(P=0.005), while there was no significant difference between those in group Band in group C(P= 0.303), and so did that between those in group Band in group D(P=0.020). Conclusion Recombinant human growth hormone (rhGH) can alleviate acute hepatic necrosis in mouse model induced by TNF-α+D-Gal, and its protective effect is relevant to the dosage administered.

    Correlation of Inflammation and Atherosclerosis in Hemodialysis Patients
    Liu Huilan;Yao Ying;Li Guogang;Zhang Xiangling;Sun Yi;Zhai Lin
    2005, 26(2):  179-182. 
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    Objective To evaluate the relationship between inflammation and atheros clerosis by measuring high sensitive C-reactive protein in hemodialysis patients' blood. Methods The study population included 52 patients with ESRD on hemodialysis(HDgroup), 14 patients who were close to the start of dialysis treatment(CRFgroup) and 30 healthy subjects(Cgroup). The high sensitive C-reactive protein(hs-CRP) concentration was detected by the latex-enhanced immunonephelometric assays. All patients and all healthy controls underwent the examination of carotid artery high-resolution B-mode ultrasound. Results The mean hs-CRPlevel was highest in hemodialysis patients among the three groups(P=0.026). The mean hs-CRPlevel was higher in patients who have carotid plaques than those who haven't carotid plaques(2.34 mg/Lversus 0.98 mg/L, P=0.04). The result of stepwise multiple logistic regression analysis for HDgroup showed that (α=0.1) only hs-CRPlevels and smoke had significant correlation in this model. The mean hs-CRPlevel was higher in patients with cardiovascular disease than control group(6.37 mg/Lversus 1.43 mg/L, P= 0.02). The result of stepwise multiple logistic regression analysis (α=0.1) to determine the independent contribution of each factor to the risk of cardiovascular disease, only hs-CRP levels showed a significant correlation in this model. Conclusion The mean CRPlevel of hemodialysis patients is increased significantly. It suggests that CRPlevel was correlated with the progression of atherosclerosis.

    Culture of Human Bone Marrow Mesenchymal Stem Cells
    Li Minghui;Tian Ding;Liu Congyan;Sun Xuejing;Wan Suigui;Su Li;Xu Juan
    2005, 26(2):  183-186. 
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    Objective To explore the culture conditions, biological characteristics and differentiation potentiality of mesenchymal stem cells(MSCs). Methods MSCs were collected and cultured ex vivo by density gradient centrifugation from human bone marrow and the expression of surface molecules and the ability of differentiation into osteoblast were examined by flow cytometric. Results MSCs could be cultured continually ex vivo; MSCs were negative on CD34, CD45, HLA-DRand CD62p, while positive on CD29, CD44, CD166 and CD90; MSCs had the potentiality of differentiating into osteoblasts. Conclusion The result suggested that MSCs derived from human bone marrow have strong reproducing ability ex vivo and can differentiate into osteoblasts in the certain condition.

    Disorders of Serum Sodium among Children after Hypothalamus Tumors Operations
    Li Dezhi;Wang Zhongcheng;Liu Ying;Zhang Yuqi;Ma Zhenyu
    2005, 26(2):  187-189. 
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    Objective To analyze characteristics of the disorders of serum sodium among children after tumors operations in the hypothalamus region. Methods 24 children with the tumor in this region were studied. The electrolyte and the sodium in 24h urine were measured everyday before and after operation. The crystal osmotic pressures of serum were analyzed at the peak of serum sodium change. Results 4 children had normal serum sodium among the 24 Cases, 6 with hypernatremia, 8 with hyponatremia and 6 with both high and low serum sodium. In hypernatremia cases, the crystal osmotic pressure of serum increased and the urine sodium reduced sharply after operation compared with that of before operation. In hyponatremia cases, the crystal osmotic pressure of serum reduced and the urine sodium increased more significantly after operation than those of before operation. Conclusion The disorders of serum sodium among children after tumors operations in the hypothalamus region were common. The disorders of serum sodium were related to the changes of urine sodium. Hypernatremia is characterized by decreased urine sodium secretion. Hyponatremia is characterized by decreased serum sodium and increased urine sodium secretion.

    Ventricular Endo-aneurysmorrhaphy and Left Ventricle Reconstruction under Ventricular Fibrillation on Hypothermia Cardiopulmonary Bypass
    Xu Dong;Jiang Long;Li Jinghong;Shang Xuebin;Liu Yanhui;Wan Feng
    2005, 26(2):  190-192. 
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    Objective To study the clinical result of ventricular endo-aneurysmorrhaphy and left ventricle reconstruction under ventricular fibrillation (VF) on hypothermia cardiopulmonary bypass. Methods Operations were performed in 14cases of ventricular aneurysm (male 12, female 2). Age range 51~75 average (63±7). NYHA Ⅳ5 cases, Ⅲ6 cases, Ⅱ2 cases, Ⅰ1 case, LVEF 10%~50%, (32±11)%. Myocardial infarction in 14 cases. 1 case was performed by direct felt strips suture under ventricular fibrillation on hypothermia cardiopulmonary bypass. 9 cases were done by endo-aneurysmorrhaphy using felt patch plus outside sandwich left ventricle reconstruction in the same way, including 3cases with thrombus removal, and 1 case with mitral valve repair. 4 cases were performed by endo-aneurysmorrhaphy using inside per-string suture plus outside sandwich left ventricle reconstruction by the same myocardial protection method, including 1case with ventricle septal perforation repair. Results Mortality of this study was zero. CPBtime was 43~168 min, (97±39) min, Ventilation time was 8~50 h, (21±14) h, 6 cases were supported by IABP. No graft for 1 case, one graft with 1 case, two grafts with 5 cases, three grafts with 5 cases, four grafts in 1 case, totally three LIMA grafts, two RAgrafts and 25 vein grafts were used in this study. One case was reopened for bleeding. Conclusion The procedure of ventricular endo-aneurysmorrhaphy under ventricular fibrillation on hypothermia cardiopulmonary bypass is safe. The result of myocardium protection of hypothermia ventricular fibrillation is satisfactory.

    Effects of IL-8, IL-10 in Acute Pancreatitis Associated with Acute Lung Injury in Rats
    Lin Dongdong;Sun Jiabang;Li Fei;Zhang Shuwen;Cui Yeqing;Liu Dachuan;Zhu Bin;Sun Haichen;Liu Shuang
    2005, 26(2):  193-196. 
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    Objective To explore the roles of IL-8 and IL-10 in pathogenesis of pancreatitis associated lung injury(PALI) in rats. Methods Pancreatitis was induced with 3.5% sodium taurocholate through of Aho et al. The severity of pancreatitis was evaluated by quantitating pancreatic pathologic score. Lung injury was evaluated by lung pathologic score and lung water content. Serum IL-8 and IL-10 were quantitated by enzyme-linked immunosorbent assay. Results Lung injury could be found at 1 h after induction of pancreatitis with 3.5% sodium taurocholate. The microscopic findings of lung injury included leukocytes infiltration in lung tissue, pulmonary sepatal thickening, pneunoedema and collapse of alveolar cavity. Lung water content increased from 4 h after induction of pancreatitis. The levels of serum IL-8 and IL-10 increased from 1 h after induction of pancreatitis, and peaked at 24 h. Conclusion Both IL-8 and IL-10 are involved in the pathogenesis of pancreatitis acute lung injury (PALI) and PALI may be the result of imbalance of systemic inflammatory response syndrome(SIRS) and compensatory anti-inflammatory response syndrome(CARS).

    Expression of Collagen TypeⅠand Repairment of Pancreatic Cell in Acute Necrotizing Pancreatitis
    Zhang Chunxia;Li Fei;Zhang Yan;Zhang Shuwen;Cui Yeqing
    2005, 26(2):  197-199. 
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    Objective To investigate the expression of collagen type Ⅰ and the effect of pancreatic cell in repairment of acute pancreatitis(AP). Methods NIH swiss rats were divided into two groups: one was the control, the other was acute pancreatitis. Acute pancreatitis were induced by intraperitoneal injection of cerulein. Routine pathological examination was used to evaluate the degree to pancreac inflammation,the expression of collagen type Ⅰ was detected by method of immunohistochemistry, and the proliferation of cell was examined by flow cytometry analysis. Results Intraperitoneal of cerulein injection could induce AP, it was the most serious duration of injury of pancreas that 8 h after cerulein injection and recovered seven days later. Expression of collagen type Ⅰ was increased in AP, and three stages of cell proliferation after APwas induced: the early active stage and the middle inhibitive stage; the last high stage. Conclusion APis a self-limited disease, which can be repaired by bodyself. Fibrosis of mesenchyma, increasing of expression of collagen type Ⅰ and proliferation of pancreatic cell play important role in the repairment of AP.

    Analysis of Death Causes of Fulminant Acute Pancreatitis
    Wang Honglu;Sun Jiabang;Chen Hong
    2005, 26(2):  200-202. 
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    Objective To investigate the death causes of patients with fulminant acute pancreatitis(FAP). Methods The clinical data of 30 d patients with FAP admitted to Xuanwu hospital from June 1992 to July 2004 were reviewed and causes of death, clinical characteristics were analyzed. FAPwas defined as presence of organ dysfunction within 72h after onset of symptoms. Results The mortality rate of FAP was 50.85%, MODS was the main cause of FAP. Mortality rate was high in FAPearly, sixteen FAPpatients died within 7d after onset of symptoms(53.33%). Conclusion The characteristic of FAP is high early mortality rate and poor prognosis. To treat MODS early and operate early are the key to reduce mortality of FAP.

    Prognostic Factors in Patients with Perforated Duodenal Ulcer Operated by Simple Closure
    Li Shengbao;Sun Jiabang;Li Fei
    2005, 26(2):  203-206. 
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    Objective To identify prognostic risk factors affecting mortality and morbidity in patients operated on perforated duodenal ulcer by simple suture. Methods Aretrospective analysis of related-prognostic factors was carried out for eighty one patients with perforated duodenal ulcer consecutively operated on by simple suture procedure in Xuanwu hospital between January 1991 and December 2003. Correlative risk factors influencing postoperative hospitalized mortality and morbidity were identified from all analytic factors. Results Postoperative mortality and morbidity were 4.2%(4/95) and 20.0%(19/95) respectively. Multivariate analysis confirmed that only preoperative shock(P=0.002) was a independent predicted mortality significantly, while time from perforation to operation(P=0.002) and concomitant medical illness(P=0.003) were both significant factors that predicted morbidity independently. Conclusion It is important that time from ulcer perforation to operation should be shorten within forty-eight hours for reducing occurrence of shock, the mortality and morbidity will be reduced following the result metioned above. Treatment for older patients with perforated duodenal ulcer should be paid more attention.

    Feasibility of Ketamine-lidocaine Anaesthetic Induction in Pediatric Patients
    Sun Yanxia;Sheng Kaihua;Li Tianzuo;Ji Fang;Zhang Bingxi
    2005, 26(2):  207-210. 
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    Objective To study the feasibility of the combination of ketamine with lidocaine for anesthesia induction in children. Methods Altogether 40 ASA physical status Ⅰ~Ⅱ children undergoing selective surgery were randomly divided into two groups of 20 each. Group Areceived ketamine and group Breceived the combination of ketamine and lidocaine for induction. Results During the induction, the changes of hemodynamics were observed,the intubation conditions were assessed as acceptable or unacceptable; the post anesthesia side-effects were recorded in all patients. The difference of cardiovascular response between two groups was significant(P<0.05). Although intubation conditions had no significant difference between two groups, laryngoscopy was easier in group Bcompared with group A(P< 0.05) and there was no coughing in group B(P<0.05). No patient in two groups reported post operation psycho-side-effects such as hallucination, but the incidence of post operation nausea and vomiting (PONV) in group Awere higher than those in group B(P<0.05). Conclusion Ketamine-lidocaine anesthetic applied for children induction has the advantages of hemodynamics stability, acceptable intubation conditions and low incidence of PONV. The combination of ketamine with lidocaine for anesthesia induction of children is suitable.

    Application of Intravenous Anesthesia with Lidocaine and Propofol Used for Neurosurgical Operation
    Li Yan;Zhou Xiaoli;Wang Baoguo
    2005, 26(2):  211-215. 
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    Objective The aim was to evaluate the feasibility of propofol and lidocaine used for neurosurgical surgery and to find out the proper dose. Methods Sixty patients were randomly allocated into four groups with different doses of lidocaine and propofol. BPand HR were controlled by nicardipine and esomolol. BIS,SEF and the hemodynamic parameters were measured continuously; the dose of nicardipine and esomolol, the recovery time and the side-effects were recorded. Results 1) The BIS and SEF values of four groups were all decreased significantly after induction. The BIS values of group Cwere lower while those of group Dwere higher than those of group A. 2) The total dose of esomolol in group Cand Dwere 43.2% and 54.4% respectively which was less than that of group A. The total dose of nicardipine in group Cand Dwere 43.4% and 54.3% respectively which was less than that of group A. Conclusion 1) Lidocaine have the power to strengthen the sedition of propofol. 2) Lidocine has the dose-dependent effect of analgesia. 3) Propofol and lidocaine are suitable for neurosurgical surgery and the proper dose is propofol 5 mg/kg and lidocaine 2.5 mg/kg.

    Correlation Between Partial CO2 Rebreathing Technique and Transesophageal Doppler for Cardiac Output Monitoring During Controlled Hypotension
    Xing Yan;Jiao Xiping;Wang Baoguo
    2005, 26(2):  216-218. 
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    Objective To evaluate the correlation between partial CO2rebreathing method and transesophageal doppler for cardiac output monitoring during controlled hypotension induced by sodium nitroprusside(SNP) in neurosurgical patients. Methods Twenty-five ASA Ⅰ~Ⅱ patients (11 male, 14 female), aged 19~55 years, undergoing elective neurosurgery were enrolled in this study. Anesthesia was maintained with 1.0 MACisoflurane. Controlled hypotension was performed after opening of dura with continuous infusion of SNP. Simultaneous measurements of cardiac output(CO) with partial CO2rebreathing method(RBCO) and transesophageal doppler(TECO) were performed in 25 neurosurgical patients. Results CO and HR increased during controlled hypotension. Agood correlation was found between RBCOand TDCO(r=0.76, P<0.01), regression equation was RBCO=0.86 TECO+0.59, with a basid calculation of -0.61 L/min. Conclusion There are good correlation between two methods. Partial CO2 rebreathing method and transesophageal doppler technique are good measurement hemodynamics for monitoring changes of COin neurosurgical patients.

    Voice Measurement with Computer and Quantitative Analysis in Normal Adult
    Yang Qing;Xu Wen;Hou Lizhen;Zhang Li
    2005, 26(2):  219-221. 
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    Objective To study the acoustic parameters of normal adults in 4 phonation modes. Methods Acoustic parameters of 60 normal adults (20~50 years old, 30 males and 30 females) were measured with computer voice evaluation system. Results The highest true voice was most precedent and the others one by one were comfortable voice, the highest falsetto voice and the lowest true voice. Jitter of comfortable voice and the lowest true voice between male and female showed statistically significant difference. Conclusion The voice of adult had distinctive characteristics with laryngeal muscle function.

    Intraoperative Blood Salvage After Blood Irradiation in Cervical Cancer
    Qi Haiyan;Deng Xiaohong
    2005, 26(2):  222-225. 
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    Objective To examine the minimum effective dosage of irradiation which can kill the cervical cancer cells in the blood. Methods Human cervical cancer cell line-HeLa cells were exposed to radiation in different dosage. The inhibitory rate of cell growth and colony were tested. HeLa cells were mixed with human red blood cells, and exposed to radiation. Then, these two kinds of cells were divided by density gradient centrifugation, and the rate of colony inhibition were tested. The blood samples were collected from 40 patients with different female malignancies, and 2,3-DPG-one of the functional indications of carrying oxygen by red blood cells, was tested after being exposed to irradiation or not. Results Either the cell growth inhibitory rate or the colony inhibitory rate of the HeLa cells was 100%(P<0.01) after being exposed to 12 Gy irradiation, meanwhile, the value of 2,3-DPG was nearly unchanged(P>0.05). Conclusion 12 Gy irradiation can kill the cervical cancer cells in the blood efficiently, and have no affection to carrying oxygen of red blood cells. The results above metioned provide the experimental data of intraoperative autotransfusion for the clinical trial in future.

    Conservative Operation with Laparoscopy for 60 Tube Pregnancy Cases
    Wang Jianjun;Li Bin;Liu Tao
    2005, 26(2):  226-228. 
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    Objective To investigate laparoscopic surgery effects for tube pregnancy. Methods The conservative operation was performed on 60 patients with tube pregnancy, fenestration on the position of tube pregnancy or taking out embryos from the tube crevice by laparoscopic surgery. Results The fallopian tubes of 60 patients were successfully retained with no complication occurred. β-HCG in blood recovered to normal level two weeks after operation, and the tube patency tests were carried out for 28 patients in one to six months after operation. The rate of oviduct opening was 64.29%. Conclusion Conservative operation of oviduct pregnancy by laparoscopy is safe and effective which is minimal invasion, less complication and quickly recovery. This method is especially suitable for the patients who want remaining bearing ability.

    综述
    The Brain Stem Escape Network Made Up of the Mauthner Cell and Other Identified Neurons
    He Zhengjuan;Li Xiaoyi;Zhang Lijuan
    2005, 26(2):  230-232. 
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    M-cells are the command neurons which active fast-start responses such as seen in fishes escaping from predatory attacks. Recently the concept of the brain stem escape network (BEN) is brought forward. Many researchers confirm the concept from different aspects with different technology. The development of the BENand forecast the direction of future study on BEN are reviewed.
    Exercise and Pregnancy
    Zhou Li;Wu Lianfang
    2005, 26(2):  233-235. 
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    Physical exercise can be benefit for our health, but the effect on pregnancy is not determined. In order to evaluate the safety of the physical exercise during pregnancy, the physiological changes, the effect on maternal and fetus and the direction and monitoring for exercise during pregnancy are reviewed.
    Nasu-Hakola Disease
    Gong Xiping;Li Yi;Wang Guoxiang
    2005, 26(2):  236-238. 
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