Abstract:

Objective To study the regulation role of dMAR on EGFPexpression. Methods The dMAR eukaryotic expression vectors were constructed by inserting 850 bp dMAR fragment into pEGFP-C1 vector at the downstream of EGFP with two different orientations. pEGFP/dMAR(+)(sense) and pEGFP/dMAR(-)(antisense) were made. The regulation of EGFP by overexpression dMAR was assessed with fluorescent microscope and flow cytometry(FCM) after the pEGFP/dMAR(+) or pEGFP/dMAR (-) vector transfected into COS7 cells. Results The EGFPexpression of pEGFP-C1, pEGFP/dMAR(+) and pEGFP/dMAR(-) was 45.1%、40.5% and 11.3% respectively 48 h post-transfection. Conclusion The expression of EGFPwas significantly inhibited by dMAR(-) co-expression. In contrast, the expression of EGFP was not affected by dMAR(+) co-expression.

Key words: EGFP, MAR, gene expression and regulation