Abstract:

Objective To explore the roles of IL-8 and IL-10 in pathogenesis of pancreatitis associated lung injury(PALI) in rats. Methods Pancreatitis was induced with 3.5% sodium taurocholate through of Aho et al. The severity of pancreatitis was evaluated by quantitating pancreatic pathologic score. Lung injury was evaluated by lung pathologic score and lung water content. Serum IL-8 and IL-10 were quantitated by enzyme-linked immunosorbent assay. Results Lung injury could be found at 1 h after induction of pancreatitis with 3.5% sodium taurocholate. The microscopic findings of lung injury included leukocytes infiltration in lung tissue, pulmonary sepatal thickening, pneunoedema and collapse of alveolar cavity. Lung water content increased from 4 h after induction of pancreatitis. The levels of serum IL-8 and IL-10 increased from 1 h after induction of pancreatitis, and peaked at 24 h. Conclusion Both IL-8 and IL-10 are involved in the pathogenesis of pancreatitis acute lung injury (PALI) and PALI may be the result of imbalance of systemic inflammatory response syndrome(SIRS) and compensatory anti-inflammatory response syndrome(CARS).

Key words: acute pancreatitis, animal model, acute lung injury, cytokine