Abstract:

Objective To explore the culture conditions, biological characteristics and differentiation potentiality of mesenchymal stem cells(MSCs). Methods MSCs were collected and cultured ex vivo by density gradient centrifugation from human bone marrow and the expression of surface molecules and the ability of differentiation into osteoblast were examined by flow cytometric. Results MSCs could be cultured continually ex vivo; MSCs were negative on CD34, CD45, HLA-DRand CD62p, while positive on CD29, CD44, CD166 and CD90; MSCs had the potentiality of differentiating into osteoblasts. Conclusion The result suggested that MSCs derived from human bone marrow have strong reproducing ability ex vivo and can differentiate into osteoblasts in the certain condition.

Key words: mesenchymal stem cells, osteoblast