Abstract:

Objective To investigate the effects of wild-type p53,GM-CSF and B7-1 genes co-transfer on proliferation ability of ovarian cancer cell line SKOV-3.Methods Transfection SKOV-3 cell with recombinant adenovirus carrying green fluorescence protein gene was used to test the transfection efficiency of adenovirus vector.Transfect SKOV-3 cell with recombinant adenovirus carrying human wild-type p53,GM-CSF and B7-1 genes,then RT-PCR was used to test the gene expression.The appearances of the cells after transfection were compared with the normal cells.Proliferation curve was performed.Results The transfection efficiency can reach 81% when the multiplicity of infection is 400 pfu/cell;the cell transfection with recombinant adenovirus carrying wild-type p53,GM-CSF and B7-1 genes can express the gene products;cell proliferation was reduced after transfection with the recombinant adenovirus carrying wild-type p53,GM-CSF and B7-1 gene.Conclusion The adenovirus vector can transduce wild-type p53,GM-CSF and B7-1 genes into ovarian cancer cell line SKOV-3 effectively;wild-type p53,GM-CSF and B7-1 genes modification can significantly reduce the proliferation of SKOV-3 cells.

Key words: gene therapy, ovarian cancer, adenovirus