Abstract: Objective To investigate the protection mechanism of β-amyloid precursor protein 17-mer peptide from UVA induced damage on cultured fibroblasts.Methods Human skin fibroblasts were irradiated with UVA.The activity of fibroblast was detected with the assay of MTT.The activity of superoxide dismutase was measured with biochemical assay.The intracellular ROS production was measured with a confocal microscopy.Results A significant decrease of activity of fibroblast was observed after exposure to UVA(P=(0.000)).A higher level of intracellular ROS(P=(0.000))and activity of superoxide dismutase(P=0.01) were detected in the irradiated samples.40 μmol/L APP17-mer peptide could counteract the effects of UVA on fibroblasts(P<0.05).Conclusion APP 17-mer peptide has potential protective effects from UVA induced damage on cultured fibroblasts.

Key words: ultraviolet rays, fibroblasts, radiation effect, reactive oxygen species, β-amyloid precursor protein 17-mer peptide