Objective To evaluate the clinical curative effect and security of therapy of Xiaozhiling injection of complete rectal prolapse.Methods 36 patients that were randomly divided into group A and B were taken double-layer four-step inject therapy.A was group of high concentration with 20 patients,and B was group of low concentration with 16 patients.The length of rectal prolapse and radiography of defecation were the indicators of curative effect,and the indicators of security were the general conditions of patients,the blood cell analysis and the function of liver and kidney.Follow-ups were taken every three months.Results In group A,19 patients were clinical controlled and 1 case was effective.In group B,16 patients were clinical controlled.There was no statistically significant effect between the two groups(P> 0.05),liver and kidney function had no serious damage.After 336 months' follow-up,7 cases recurred.Of the 7 patients,4 were clinical controlled after the second time injection,1 was taken intestine resection,1 was taken Sling fixation of rectum,1 was taken PPH.Conclusion The therapy is painless,effective,simple,low-cost and repeatable.Which is appropriate for being generalized in the grass roots hospitals as preferred method.