Journal of Capital Medical University ›› 2006, Vol. 27 ›› Issue (6): 771-773.

• 基础研究 • Previous Articles     Next Articles

The Changes of Toll-like Receptor mRNA Expression in Human Umbilical Vein Endothelial Cells after Lower Laminar Shear Stress

Liang Feng1, Hu Dayi2, Wu Mingying2, Li Tianchang2, Shi Xubo2, Tang Chuzhong2, Wang Boyao3, Huang Ning3, Wu Qi3, Liu Xuqing4   

  1. 1. Department of Cardiology, Daxing District People's Hospital;2. Cardiovascular Center, Beijing Tongren Hospital, Capital University of Medical Sciences;3. West China Medical Center, Sichuan University;4. Beijing Xicheng District Pingan Hospital
  • Received:2005-05-08 Revised:1900-01-01 Online:2006-12-24 Published:2006-12-24

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Abstract: Objective By using the technology of RT-PCR and Northern hybridization,to assess the expression of TLR-2 and TLR-4 in human umbilical vein endothelial cells(HUVECs) after lower laminar shear stress.Methods Total RNA was isolated from HUVECs untreated and treated with 42 μN/cm2 for 1 h.The technology of RT-PCR and Northern hybridization were used to determine the change of TLR-2 and TLR-4 mRNAs expression after 1 hour stimulation of laminar shear stress.Results The results of RT-PCR and Northern hybridization showed that the expression of TLR-4 in HUVECs was increased after 1 h shear stress.There was no change for TLR-2 after stimulation.Conclusion Lower laminar shear stress can induce TLR-4 mRNA expression.The experiment suggests that the inflammatory signal receptor TLR-4 would probably be involved in flow shear stress-induced effective gene expression in human vascular endothelial cells.

Key words: human umbilical vein endothelial cells, shear stress, Toll like receptor

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