Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (3): 346-351.

• 传染病及相关慢性疾病研究进展 • Previous Articles     Next Articles

Expression profiling of Mycobacterium tuberculosis Beijing genotype invadingTHP-1 cell line

LI Wei-min1, HUANG Hai-rong1, GAO Feng3, LIU Yi2, FAN Wei-xing3, DAI Guang-ming1, JIANG Guang-lu1, SONG Xiao-yun1, ZHAO Li-ping1, FU Yu-hong1
  

  1. 1. Chinese Tuberculosis Clinical Lab, Beijing Chest Hospital, Beijing 101149, China; 2. Department of Immunology and Bacteriology,Beijing Chest Hospital, Beijing 101149, China; 3. China Animal Health and Epidemiology Center, Qingdao 266032, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-06-21 Published:2011-06-21

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Abstract: Objective To explore immunologic mechanism in pathogensis relevant to tuberculosis(TB), especially caused by Mycobacterium tuberculosis(M.tb) Beijing genotype by analyzing the different expression profile of cells infected.
Methods IS6110 DNA RFLP and Spoligotyping patterns of 265 M.tb Beijing Genotype isolates from a nationwide random survey for the epidemiology of TB in China in 2000 were calculated using the average linkage to get a central strain of M.tb Beijing genotype (referred to as M. tb Beijing genotype). THP-1 cells were infected with M.tb, M.bovis and M. tb Beijing genotype, the expression profile was analyzed using gene chips. Furthermore the results were evaluated by MAS system.
Results The central strain of M.tb Beijing genotype was determined on the maximum coefficient(0.514) by the formula. There were some different expression genes(tnf, tnfsf 9, pim-1, icam-1 and cd48) by THP-1 cell infected between M.tb H37Rv and M. tb Beijing genotype.
Conclusion The central strain of M.tb Beijing genotype was of Chinese representation. The result of microarray analysis of the infected cells was reliable and analyzable; 293 genes were common expressed from the THP-1 cell infected by M.tb H37Rv, M.bovis and the M. tb Beijing genotype.

Key words: mycobacterium tuberculosis, Beijing genotype, gene microarray, expression profile

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