Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (3): 361-364.

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Relationship between mitochondrial α-synuclein contents and oxidative stress levels in the rat brain

SUN Jiu-yan, LI Xin, LIU Guang-wei, CHENG Fu-rong, LI Yao-hua, YU Shun*   

  1. Department of Neurobiology, Beijing Institute of Geriatrics, Xuanwu Hospital, Capital Medical University,Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Beijing100053, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-06-21 Published:2011-06-21
  • Contact: YU Shun

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Abstract:

Objective To study the relationship between mitochondrial α-synuclein(α-SYN) contents and oxidative stress levels in different rat brain regions and its association with selective damage of dopaminergic neurons in patients with Parkinson’s disease.
Methods Mitochondria were isolated using Percoll gradient centrifugation and mitochondrial α-SYN contents were measured using Western blotting and ELISA. Oxidative stress levels were evaluated by measuring oxidative malonaldehyde(MDA) and anti-oxidative indices total anti-oxidation capability(T-AOC), glutathione(GSH), glutathione peroxidase(GSH-PX) and superoxide dismutase(SOD) in brain tissues.
Results α-SYN contents were higher in PD vulnerable area such as olfactory bulb, striatum and hippocampus than in frontal cortex and cerebellum.
Conclusion Mitochondrial α-SYN contents are different in various rat brain regions but it is not related to oxidative levels.

Key words: brain, rat, oxidative stress, mitochondria, &alpha, -synuclein

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