Distinct sensitivity of different arterial baroreceptors on ruthenium red

doi:10.3969/j.issn.1006-7795.2014.05.024

Journal of Capital Medical University ›› 2014, Vol. 35 ›› Issue (5): 640-645.doi: 10.3969/j.issn.1006-7795.2014.05.024

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Distinct sensitivity of different arterial baroreceptors on ruthenium red

Zhao Haiyan¹, Huang Haixia², Liu Ping², Zhang Sitao², Niu Weizhen², Wang Wei²

1. Department of Medical Functional Science, Yanjing Medical College, Capital Medical University, Beijing 100069, China;
2. Department of Physiology and Pathophysiology, School of Basic Medical Sciences, Capital Medical University, Beijing 100069, China

Received:2014-03-05 Online:2014-10-21 Published:2014-10-20
Supported by:
This study was supported by Beijing Municipal Commission of Education (PXM2007-014226-050086).

Abstract

Abstract:

Objective To investigate if ruthenium red (RR) possesses differentiating effects on baroreceptors and to understand the relationship between these different effects and types of the baroreceptors. Methods Carotid sinus-nerve preparation of the rabbit was perfused intrasinusally and extrasinusally in vitro. Baroreceptor discharges were recorded from nerve filaments containing 2-5 fibers while the intrasinusal pressure was clamped with pulsatile or ramp waves. Single fiber discharges were obtained by sorting the few-fiber discharge using software Spike 2. Results According to the response to RR (20 μmol /L), baroreceptor fibers can be distinguished into two types: RR sensitive and insensitive fibers. To further analyze electrophysiological characteristics of the two types, we found that the RR sensitive and insensitive fibers correspond with C- and A-fibers, respectively.Conclusion Carotid sinus fibers can be classified into two types according to the response to RR (20 μmol /L). The two types of baroreceptors might belong to A- and C-fibers, respectively. The results suggested that the mechanisms for mechano-electrical transduction in the two types of baroreceptor fibers might not be the same.

Key words: baroreceptor, ruthenium red, ruthenium red sensitive fibers

CLC Number:

R329.3

Zhao Haiyan, Huang Haixia, Liu Ping, Zhang Sitao, Niu Weizhen, Wang Wei. Distinct sensitivity of different arterial baroreceptors on ruthenium red[J]. Journal of Capital Medical University, 2014, 35(5): 640-645.

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Distinct sensitivity of different arterial baroreceptors on ruthenium red

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