Ability of Omicron variant of SARS-CoV-2 to utilize angiotensin-converting enzyme Ⅱ from different animals as receptors for cellular entry

doi:10.3969/j.issn.1006-7795.2022.03.015

Journal of Capital Medical University ›› 2022, Vol. 43 ›› Issue (3): 421-426.doi: 10.3969/j.issn.1006-7795.2022.03.015

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Ability of Omicron variant of SARS-CoV-2 to utilize angiotensin-converting enzyme Ⅱ from different animals as receptors for cellular entry

Qiu Yaruo^1#, Li Xinglin^2#, Chen Danying², Liu Yongmei², Song Yanjun², Li Guoli², Song Chuan², Wang Xi², Zhao Xuesen^1*

1. Peking University Ditan Teaching Hospital, Beijing 100015, China;
2. Institute of Infectious Diseases, Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China

Received:2022-03-20 Online:2022-06-21 Published:2022-06-01
Contact: *E-mail:zhaoxuesen@ccmu.edu.cn
Supported by:
National Natural Science Foundation of China (81971916).

Abstract

Abstract: Objective To investigate the ability of Omicron variant of SARS-CoV-2 to utilize angiotensin-converting enzyme Ⅱ(ACE2) from different animals as receptors for cellular entry, and to explore the potential cross-species transmissibility of Omicron. Methods Plasmids encoding ACE2 molecules from different animals were constructed and transfected into 293T cells. The pseudoviral infection systems of D614G strain, Delta strain and Omicron strain were established to determine receptor activity of different animals’ ACE2 for cellular entry. The utilization ability of Omicron strain to ACE2 receptors were examined by luciferase assay. Results ACE2 from different animals expressed in 293T cells supported cellular entry mediated by spike protein. Compared with D614G, the ability of Omicron-pp to invade cells using mouse ACE2 molecules was significantly increased (t=16.09, P<0.05), while it decreased when using ACE2 molecules from Chinese horseshoe bat, Mexican free-tailed bat, Ferret badger, Hog badger, Feline, Rabbit, or Pangolin (t=17.80, P<0.05; t=8.43, P<0.05; t=18.10, P<0.05; t=10.46, P<0.05; t=22.00, P<0.05; t=10.08, P<0.05; t=4.83, P<0.05, respectively). Compared with Delta, the ability of Omicron-pp to entry cells was decreased when using Chinese horseshoe bat, Ferret badger, Hog badger and Feline (t=8.15, P<0.05; t=7.91, P<0.05; t=8.59, P<0.05; t=8.43, P<0.05, respectively). Conclusion The ACE2 molecules from different animals served as functional receptors for cellular entry mediated by Omicron spike protein. Our findings herein suggest that there may exist a risk of multiple cross-species transmission of Omicron among different mammal animals.

Key words: severe acute respiratory syndrome coronavirus 2 (SARS-Cov-2), Omicron, angiotensin-converting enzyme Ⅱ

CLC Number:

R563.1

Qiu Yaruo, Li Xinglin, Chen Danying, Liu Yongmei, Song Yanjun, Li Guoli, Song Chuan, Wang Xi, Zhao Xuesen. Ability of Omicron variant of SARS-CoV-2 to utilize angiotensin-converting enzyme Ⅱ from different animals as receptors for cellular entry[J]. Journal of Capital Medical University, 2022, 43(3): 421-426.

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Ability of Omicron variant of SARS-CoV-2 to utilize angiotensin-converting enzyme Ⅱ from different animals as receptors for cellular entry

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