Regulation of unilateral stimulating striatal D1-MSN with different frequencies on movement in mice

doi:10.3969/j.issn.1006-7795.2025.02.014

Abstract

Abstract: Objective To clarify the effect of unilateral activation of the dopamine type I receptor medium-sized multi-spiny neurons (D1-MSN) in the dorsal striatum of mice on speed. Methods The transgenic animals were combined with optogenetic experiments to specifically activate the D1-MSN in the dorsal striatum of mice at different frequencies and to analyze the rotational behavior and speed of mice when stimulating the D1-MSN. Results Unilateral activation of D1-MSN induces contralateral rotational behavior in mice and either increases or decreases speed. The mechanisms by which different frequencies affect the speed of mice differently. As the frequency of stimulus increased, the contralateral rotational behavior of the mice increased. Unilateral stimulus of D1-MSN increased speed and induced contralateral rotational behavior, and the rotational behavior increased with increasing stimulus frequency. In the experiment where D1-MSN stimulus did not induce rotational behavior, it was found that 5 Hz stimulus still induced an increase in speed, but 15 Hz and 25 Hz stimulus did not induce an increase in speed. Further analysis of the pre-stimulus locomotor state of the mice showed that 5 Hz, 15 Hz and 25 Hz stimulus increased speed when the average speed before stimulus was less than 5 cm/s. However, 15 Hz stimulus decreased the speed when the average speed before stimulus was greater than 5 cm/s. Conclusion Unilateral activation of D1-MSN in the dorsal striatum modulates speed and induces contralateral rotational behavior in mice, and is related to the frequency of stimulus and the locomotor state of the mice before stimulus.

Key words: basal ganglia loops, striatum, medium spiny neurons, rotational behavior, velocity, mouse

CLC Number:

R339.4

Cheng Fangyuan, Chen Dongkun, Liu Huijin, Jia Jun, Wang Ke. Regulation of unilateral stimulating striatal D1-MSN with different frequencies on movement in mice[J]. Journal of Capital Medical University, 2025, 46(2): 283-288.

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