Abstract: Objective To evaluate the advantages and disadvantages of methods of ProCOUNT and single-platform ISHAGE gating strategy for CD34 positive cells in peripheral blood and apheresis samples.Methods Flow cytometry was adopted to enumerate CD34 positive cells in peripheral blood and apheresis samples with ProCOUNT and single-platform ISHAGE gating strategy.And enumeration of CD34 positive cells in 23 peripheral blood and 50 apheresis samples were performed.The results of the two methods were compared using Spearman correlation and paired t-test.Results There was a strong correlation between the absolute counting and percentage of CD34 positive cells for the two methods(r all >0.9,P all<0.001).The absolute counting in peripheral blood and apheresis samples with ProCOUNT and single-platform ISHAGE gating strategy were(42.7±7.5) μL^-1 and(52.1±7.2) μL^-1(P<0.01)respectively,(30515.2±684.7) μL^-1 and((30923.8)±711.1) μL^-1(P<0.01)respectively.The percentage of CD34 positive cells in peripheral blood and apheresis samples were(0.26±0.07)% and(0.13±0.05)%(P<0.01)respectively,(0.79±0.18)% and(0.83±0.17)%(P=0.056)respectively.All of the results using ProCOUNT were lower than those using single-platform ISHAGE gating strategy.Conclusion There is a strong correlation between the absolute counting and percentage of CD34 positive cells for the ProCOUNT and single-platform ISHAGE gating strategy,but the result of the former is significantly lower than that of the latter.

Key words: ProCOUNT, ISHAGE, flow cytometry, CD34~+ cell