Abstract:

The present study proved that Escherichia coli synthesized ademolybd-precursor of Formate dehychogenase (FDH) in culture media containing tungstate under anaerobic condition. The whole cell conld restore FDH activity when tungstate was removed and molybdate was added. But purified demolybdo-enzyme would not restore its activity under he same condition. The experiment provides a useful material for the study of metalion incorporateing into apoenzyme.