Change of α-Synuclein Level in Rat Brain and Plasma after Ischemia/Reperfusion

doi:10.3969/j.issn.1006-7795.2010.03.017

Journal of Capital Medical University ›› 2010, Vol. 31 ›› Issue (3): 368-372.doi: 10.3969/j.issn.1006-7795.2010.03.017

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Change of α-Synuclein Level in Rat Brain and Plasma after Ischemia/Reperfusion

JIA Chun-song¹, LI Xin¹, LI Yao-hua¹, LIU Guang-wei¹, YIN Juan-juan¹, WU Yan-chuan², YU Shun^1*

1. Department of Neurobiology of Beijing Institute of Geriatrics, Key Laboratory for Neurodegenerative Diseases of Ministry of Education, Xuanwu Hospital, Capital Medical University; 2. Central Laboratory, Xuanwu Hospital, Capital Medical University

Received:1900-01-01 Revised:1900-01-01 Online:2010-06-21 Published:2010-06-21
Contact: YU Shun

Abstract

Abstract:

Objective To explore the change of α-synuclein(α-Syn) in rat brain and plasma after ischemia/reperfusion. Methods Thirty adult male Wistar rats were randomly divided into 2 groups: sham operation group(10 cases) and ischemia/reperfusion group(20 cases). A model of middle cerebral artery occlusion(MCAO) was induced by the intraluminal suture for the latter group. After 1.5 h ischemia, reperfused for 24 h, then the level of α-Syn in the plasma and penumbra, infarct core and their controls were measured by Western blotting and ELISA method. Results The level of α-Syn was increased significantly in the penumbra, but did not change obviously in the infarct core or plasma. Conclusion α-Syn was increased in the penumbra of brain after ischemia/reperfusion.

Key words: &alpha, -synuclein, ischemia/reperfusion, penumbra, infarct core, plasma

CLC Number:

R 743

JIA Chun-song, LI Xin, LI Yao-hua, LIU Guang-wei, YIN Juan-juan, WU Yan-chuan, YU Shun. Change of α-Synuclein Level in Rat Brain and Plasma after Ischemia/Reperfusion[J]. Journal of Capital Medical University, 2010, 31(3): 368-372.

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Change of α-Synuclein Level in Rat Brain and Plasma after Ischemia/Reperfusion

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