Journal of Capital Medical University ›› 2006, Vol. 27 ›› Issue (3): 355-357.

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Study on the Acetylcholine System in the Brain of Non-Insulin-Dependent Diabetes Mellitus Animal Model, KK-Ay Mice

Wang Xianling1, Jia Jianping1, Zuo Pingping2, Yang Nan2   

  1. 1. Neurology Department, Xuanwu Hospital, Capital University of Medical Sciences;2. Pharmacy Department of Peking Union Medical College
  • Received:2005-07-29 Revised:1900-01-01 Online:2006-06-24 Published:2006-06-24

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Abstract:

Objective To investigate the pathogenetic mechanism of diabetic encephalopathy..Methods The non-insulin-dependent diabetes mellitus (NIDDM) animal models, KK-Ay mice, were used. Their cognitive performance and brain structure were studied. The activity of choline acetyltransferase (ChAT) was assayed with 3 H-CoA as the substrate and the binding activity of radiolabelled 3 H-QNB for acetylcholine muscarinic receptors (Mreceptors) was investigated in the cerebral cortex and hippocampus of KK-Ay mice..Results Cognitive impairment and changes of brain structure of NIDDM mice were found. In the cerebral cortex and hippocampus of KK-Ay mice, the activity of choline acetyltransferase (ChAT) was significantly reduced at 12 weeks of duration compared with that of age-matched controls. The binding activity of 3 H-QNB for M receptor in the diabetic mice was not significantly different from that of the controls..Conclusion The acetylcholine system deficits are closely related to the cognitive impairment of diabetic mice.

Key words: diabetic encephalopathy, choline acetyltransferase(ChAT), M receptor

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