Clonging and Expression for DNA Fragment of Complete Sequence and Catalytic Domain of Human Tyrosine Hydroxylase

Journal of Capital Medical University ›› 2000, Vol. 21 ›› Issue (4): 277-280.

• 论著 • Previous Articles Next Articles

Clonging and Expression for DNA Fragment of Complete Sequence and Catalytic Domain of Human Tyrosine Hydroxylase

Xiong Ying¹, Liu Hua¹, Yu Peilan¹, Zhang Chengbo¹, Yang Hui², Xu Qunyuan²

1. Department of Biochem, Capital University of Medical Sciences;2. Beijing Institute for Neuroscience

Received:2000-05-17 Revised:1900-01-01 Online:2000-10-15 Published:2000-10-15

Abstract

Abstract: In order to obtain complete sequence and catalytic core of human tyrosine hydroxylase(TH) cDNA,the full length of cDNA from human TH was used as template and the specific oligonucleotide primers were designed for PCR.The target fragments were cloned into vector pGEX-4T-1 respectively.The recombinant plasmids were transferred into E.coli BL21,and the expression of target protein was induced with IPTG.The expressed protein was analysed by SDS-PAGE and western-blotting.The result showed that the cDNA fragments of complete sequence and catalytic core of human TH were harvested.The sequencing for them showed that the cloned target gene fragments were correct.SDS-PAGE indicated that the proteins with Mr about 83000 and 66000 were expressed with the recombinant plamids pGEXTHa and pGEXTHc respectively.

Key words: tyrosine hydroxylase, expression, catalytic core, complete seque

CLC Number:

Q546

Xiong Ying;Liu Hua;Yu Peilan;Zhang Chengbo;Yang Hui;Xu Qunyuan. Clonging and Expression for DNA Fragment of Complete Sequence and Catalytic Domain of Human Tyrosine Hydroxylase[J]. Journal of Capital Medical University, 2000, 21(4): 277-280.

[1]	Zhang Jianxiong, Li Dandan, Hu Mengmeng, Cheng Xuewei, Dong Ruihua. Relationship between carboxylesterase 1 and carboxylesterase 2 gene expression levels and body mass index, and gene polymorphism analysis [J]. Journal of Capital Medical University, 2023, 44(1): 13-19.
[2]	Su Wenting, Yu Jianan, Jia Jun, Wang Xiaomin. Electroacupuncture selectively regulates the expression of c-Fos in the dorsal striatum of Parkinsonian mice [J]. Journal of Capital Medical University, 2021, 42(6): 986-992.
[3]	Liu Jun, Cao Qingfei, Ye Xiongjun, Chen Weinan, Zhao Haiyue, Tong Ming, Wang Yinyin, Huang Xiaobo, Chang Zhijie. Relationship between CREPT expression and prognosis in renal cell carcinoma [J]. Journal of Capital Medical University, 2020, 41(4): 552-557.
[4]	Yao Jiannan, Gao Tianbo, Duan Ling, Liu Jian, Jiang Yuliang, An Guangyu, Ge Yang. Screening and analysis of O-glycosylation-associated differential expressed genes in colorectal cancer [J]. Journal of Capital Medical University, 2020, 41(3): 403-410.
[5]	Xu Wenjian, Li Wei. Building a prediction model of brain tissues gene expression based on whole blood gene expression profiles [J]. Journal of Capital Medical University, 2019, 40(5): 731-737.
[6]	Niu Huan, Cheng Shan, Ding Wei. The 2018 Lasker Awards(Ⅰ)-Basic Medical Research and Special Achievement in Medical Science [J]. Journal of Capital Medical University, 2018, 39(5): 778-782.
[7]	Bi Kuo, Qin Jiajia, Yang Jing, Zhu Xinping. Immunoscreening and identification of a novel full-length cDNA encoding membrane protein of Trichinella spiralis [J]. Journal of Capital Medical University, 2015, 36(2): 232-238.
[8]	Fa Pengyan, Liu Hua, Zheng Junfang, Wang Lei, Dai Yuanping, He Junqi. NHERF3 expression level and its significance in renal cell carcinoma [J]. Journal of Capital Medical University, 2015, 36(2): 251-254.
[9]	YIN Ai-hong;WU Wen-qi;CUI Shi-jun;TENG Xu;ZHENG Shao-peng. Experimental study of hypoxia-inducible expression vector carrying VEGF gene on rabbit limb ischemia models [J]. Journal of Capital Medical University, 2012, 33(3): 356-360.
[10]	WU Wen-qi;CONG Xu-zhen;YIN Ai-hong;HU Jia;ZHAI Fang-li;LI Shen-tao. Expression, purification and characterization of ribose 5-phosphate isomerase A from Streptococcus mutans [J]. Journal of Capital Medical University, 2012, 33(2): 227-232.
[11]	LIU Yang;ZHAO Yong-mei;ZHANG Hai-yan;LI Wei-hong;YUE Yun. Effects of overexpression of nurr1 on apoptosis of SK-N-SH cells induced by neurotoxin 6-OHDA [J]. Journal of Capital Medical University, 2011, 32(4): 525-533.
[12]	LI Wei-min;HUANG Hai-rong;GAO Feng;LIU Yi;FAN Wei-xing;DAI Guang-ming;JIANG Guang-lu;SONG Xiao-yun;ZHAO Li-ping;FU Yu-hong. Expression profiling of Mycobacterium tuberculosis Beijing genotype invadingTHP-1 cell line [J]. Journal of Capital Medical University, 2011, 32(3): 346-351.
[13]	LIU Xiao-juan△;ZHANG Li-qing△;LI Jian;ZHAI Fang-li;LI Shen-tao;. Cloning, Expression and Purification of Human Synuclein Gamma [J]. Journal of Capital Medical University, 2011, 32(2): 254-258.
[14]	SHI Ying;LIU Ning;JI Yun-xia;WEI Zheng;ZHANG Tong;CHEN De-xi;LOU Jin-li. Relationship between CCR5 Receptor Expression Levels of CD4⁺T Lymphocytes in Peripheral Blood and HIV Infection [J]. Journal of Capital Medical University, 2010, 31(6): 711-714.
[15]	XING Feng-ling;CHEN Xue-qin;FENG Li-min;ZHANG Hua. Expression of Tumor Suppressor Gene PTEN in Endometrium of Postmenoposal Women with Breast Cancer Treated with Tamoxifen [J]. Journal of Capital Medical University, 2010, 31(3): 405-408.

Clonging and Expression for DNA Fragment of Complete Sequence and Catalytic Domain of Human Tyrosine Hydroxylase

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments