Abstract: The DNA sequence coding for human Vascular Endothelial Growth Factor (hVEGF) was inserted into the down stram of thioredoxin gene in pET32M to generate thioredoxin-VEGF fusion protein expression vector pET32M/V. After identification and transformation, the thioredoxin-VEGF fusion protein with the molecular weight of 32000 was detected by SDS-PAGE analysis and Western blotting after induced expression in E. coli BL21. The recombinant proteins were recovered as inclusion bodies, but there were also some soluble recombinant proteins. Chick Chorioallantoic Membrane (CAM) bioassay showed that recombinant protein had biological activities of hVEGF.

Key words: vascular endothelial growth factor, fusion protein, induced expression