Journal of Capital Medical University ›› 2012, Vol. 33 ›› Issue (5): 643-646.doi: 10.3969/j.issn.1006-7795.2012.05.018

• 基础研究 • Previous Articles     Next Articles

Effects of triptolide on expression of inducible nitric oxide synthase and production of nitrogen monoxide in microglia cells induced by β-amyloid

WANG Hui-ling1, ZHOU Xiao-chun1, MA Chun-hu1, WANG Bing2, WANG Xiao-min3   

  1. 1. Physiological Teaching and Research Section, Chengde Medical College, Chengde 067000, Hebei Province, China;2. Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province,China;3. Capital Medical University, Beijing 100069 China
  • Received:2012-09-19 Revised:1900-01-01 Online:2012-10-21 Published:2012-10-21

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Abstract: Objective To investigate the effects of triptolide (T10) on expression of inducible nitric oxide synthase (iNOS) and production of nitrogen monoxidum (NO) in microglia cells induced by β-amyloid(Aβ)1-42. Methods The microglia cells were pre-treated with 10-11-10-8 mol/L T10 for 12 h before 10 μmol/L Aβ1-42 were added, and incubated for 12 h together. The production of NO was determined by Griess reaction, and the expression of iNOS was determined by Western blotting. Results Expression of iNOS and production of NO in Aβ group were significantly higher than those in control group(P<0.01). T10 treatment reduced expression of iNOS and production of NO obviously induced by Aβ from microglia cells(P<0.05).Conclusion T10 significantly inhibited expression of iNOS and production of NO in microglia cells induced by Aβ. T10 might provide a novel therapy for AD.

Key words: Alzheimer's disease, triptolide, microglia cell, nitrogen monoxidum, inducible nitric oxide synthase

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