Effect of remote ischemic preconditioning on inducible nitric oxide synthase expression following focal cerebral ischemia in rat

doi:10.3969/j.issn.1006-7795.2014.03.011

Abstract

Abstract:

Objective The objective of this study is to investigate the effect of remote ischemic preconditioning(RIPC) on the expression of inducible nitric oxide synthase(iNOS) in ischemia-reperfusion rats to explore the relative mechanisms of RIPC on cerebral ischemic injury. Methods Middle cerebral artery occlusion(MCAO) operation was performed using suture method. Twenty-one male Sprague Dawley rats were divided randomly into three groups: sham group(n=7), MCAO group(n=7) and RIPC+MCAO group(n=7). Three cycles of RIPC induced by temporarily occluding the bilateral femoral arteries(10 minutes) prior to 10 minutes of reperfusion were given three times a day for 3 days before the animal received MCAO surgery. After 2 h ischemia and 24 h reperfusion, neurological deficits were evaluated and brain infarct volume was measured by 2, 3, 5-triphenyltetrazolium chloride(TTC) staining. The expression of iNOS in ipsilateral hemisphere was determined quantitatively by Western blotting analysis. Results 1 The physiological parameters of the three groups of rats were in normal arrange showing no statistically significant difference. The neurological deficit score and brain infarct volume in MCAO group rats increased significantly compared with those of sham group at 24 h after reperfusion(P<0.05), which were significantly decreased by RIPC+MCAO treatment(P<0.05). 2 The expression of iNOS in ipsilateral hemispheres of MCAO group rats at 24 h after reperfusion increased significantly compared with that of sham group rats(P<0.05) while the expression of iNOS in RIPC+MACAO group reduced remarkably compared with that of MCAO group(P<0.05). Conclusion RIPC treatment protects against cerebral ischemic injury in rats, which might be related to the decreased expression of iNOS in the ischemic brain.

Key words: remote ischemic preconditioning, cerebral ischemia, inducible nitric oxide synthase

CLC Number:

R743.31

Yan Feng, Yin Jie, Luo Yumin, Li Sen, Wang Yulan, Zhao Yongmei. Effect of remote ischemic preconditioning on inducible nitric oxide synthase expression following focal cerebral ischemia in rat[J]. Journal of Capital Medical University, 2014, 35(3): 315-319.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2014.03.011

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2014/V35/I3/315

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