Association between lactobacillus species and bacterial vaginosis associated bacteria in non-pregnant women

doi:10.3969/j.issn.1006-7795.2013.04.011

Journal of Capital Medical University ›› 2013, Vol. 34 ›› Issue (4): 535-539.doi: 10.3969/j.issn.1006-7795.2013.04.011

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Association between lactobacillus species and bacterial vaginosis associated bacteria in non-pregnant women

CHEN Jianing¹, LV Zhi¹, SU Jianrong¹, KANG Xixiong²

1. Center of Clinical Laboratory, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China;
2. Laboratory Diagnosis Center, Beijing Tiantan Hospital, Capital Medical University, Beijing 100050, China

Received:2013-02-20 Online:2013-08-21 Published:2013-07-20
Supported by:
This study was supported by National Natural Science Foundation of China (30972819).

Abstract

Abstract:

Objective To evaluate the association between popular lactobacillus species and bacterial vaginosis(BV) associated bacteria in vaginal flora. Methods Vaginal fluid samples were taken from 170 women, classified as BV(n=86) or non-BV(n=84) using the Amsel criteria and Nugent score system. Genomic DNA extracted from these samples was analyzed for bacterial sequences of three Lactobacillus species(L. crispatus, L. jensenii and L. iners) and five BV associated bacteria(G.vaginalis, A. vaginae, prevotella sp, megasphaera sp and leptotrichia sp) by 16S rRNA PCR. Results The prevalence of Lactobacillus species was significantly higher in the non-BV group than in the BV group, the BV associated bacteria vice versa. Women without L. jensenii were significantly more likely to have G. vaginalis, A. vaginae and leptotrichia sp, without L. iners more likely to have G. vaginalis, without L. crispatus likely to have prevotella sp. Five BV associated bacteria coexisted with each other except that there was no significant association between megasphaera sp and G. vaginalis. Conclusion Not only normal vaginal flora, BV vaginal flora was complicated also because many different BV associated bacteria coexisted. The absence of L. jensenii or L. iners may influence the prevalence of BV associated bacteria.

Key words: vaginal microbial flora, bacterial vaginosis, lactobacillus, molecular diagnosis

CLC Number:

R711.31

CHEN Jianing, LV Zhi, SU Jianrong, KANG Xixiong. Association between lactobacillus species and bacterial vaginosis associated bacteria in non-pregnant women[J]. Journal of Capital Medical University, 2013, 34(4): 535-539.

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Association between lactobacillus species and bacterial vaginosis associated bacteria in non-pregnant women

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