Abstract: Objective To develop a novel preparation of liposomal doxorubicin. Methods The liposome was prepared by dispersion film assay, loaded doxorubicin by ammonium sulfate gradient method, the anti-proliferation activities of doxorubicin liposome and doxorubicin against cancer cells evaluated by MTT assay and the antitumor activities of doxorubicin liposome and doxorubicin on S180 mouse model were measured. Results The doxorubicin liposome encapsulation efficiency was more than 96.3% at different time points. Doxorubicin liposome effectively inhibited the proliferation of carcinoma cells. The in vivo anti-tumor activity and toxicity of doxorubicin liposome were significantly higher and lower than that of doxorubicin, respectively. Conclusion The method is practicable to prepare doxorubicin liposome having good antitumor potency in vivo.

Key words: doxorubicin liposome, cytotoxicity, releasing curve, anti-tumor activity in vivo