Differential expressions of oligomer α-synuclein and phosphorylated α-synuclein in synaptosome in the brain regions of different ages of cynomolgus monkeys of different ages

doi:10.3969/j.issn.1006-7795.2015.05.001

Journal of Capital Medical University ›› 2015, Vol. 36 ›› Issue (5): 671-674.doi: 10.3969/j.issn.1006-7795.2015.05.001

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Differential expressions of oligomer α-synuclein and phosphorylated α-synuclein in synaptosome in the brain regions of different ages of cynomolgus monkeys of different ages

Yang Weiwei^1,2, Li Xin^1,2, Li Xuran^1,2, Li Xuying^1,2, Yu Shun^1,2,3

1. Department of Neurobiology, Xuanwu Hospital, Capital Medical University, Beijing 100053, China;
2. Key Laboratory of Neurodegenerative Disease, Ministry of Education, Beijing 100053, China;
3. Center for Parkinson's Disease, Beijing Institute for Brain Disorders, Beijing 100053, China

Received:2015-09-14 Online:2015-10-21 Published:2015-10-20
Contact: 于顺 E-mail:yushun103@163.com
Supported by:
This study was supported by Major State Basic Research Development Program of China (973 Program)(2011CB504101), Natural Science Foundation of China (81071014, 81371200, 81401042), National Science and Technology Support Program (2012BAI10B03), Natural Science Foundation of Beijing (7122035), The Capital Health Research and Development Special Fund (2011-4001-01).

Abstract

Abstract: Objective To explore the expressions of oligomer α-synuclein (oligo-α-syn) and phosphorylated α-synuclein (p-α-syn) in synaptosome in brain regions of cynomolgus monkeys of different ages. Methods ELISA was applied to measure the expressions of oligo-α-syn and p-α-syn in different brain regions of cynomolgus monkeys of different ages. Results The synaptosome-α-Syn oligomers and synaptosome-p-α-syn increased significantly in prefrontal lobe, occipital lobe, temporal lobe, hippocampus, striatum and thalamus of old monkeys compared with that of young and middle-aged monkeys (P<0.01). Conclusion The highest level expression of oligo-α-syn and p-α-syn was found in synaptosome of all brain regions in old cynomolgus monkeys.

Key words: oligomer α-synuclein (oligo-α-syn), phosphorylation-α-synuclein (p-α-Syn), cynomolgus monkeys, synaptosome

CLC Number:

R338

Yang Weiwei, Li Xin, Li Xuran, Li Xuying, Yu Shun. Differential expressions of oligomer α-synuclein and phosphorylated α-synuclein in synaptosome in the brain regions of different ages of cynomolgus monkeys of different ages[J]. Journal of Capital Medical University, 2015, 36(5): 671-674.

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References

[1] Lin C H, Chen M L, Tai Y C, et al. Reaffirmation of GAK, but not HLA-DRA, as a Parkinson's disease susceptibility gene in a Taiwanese population [J]. Am J Med Genet B Neuropsychiatr Genet, 2013, 162B (8): 841-846.
[2] Mizuta I, Takafuji K, Ando Y, et al. YY1 binds to alpha-synuclein 3,-flanking region SNP and stimulates antisense noncoding RNA expression [J]. J Hum Genet, 2013, 58 (11): 711-719.
[3] Brockmann K, Schulte C, Hauser A K, et al. SNCA: major genetic modifier of age at onset of Parkinson's disease [J]. Mov Disord, 2013, 28 (9): 1217-1221.
[4] Chang C, Lang H, Geng N, et al. Exosomes of BV-2 cells induced by alpha-synuclein: important mediator of neurodegeneration in PD [J]. Neurosci Lett, 2013, 548: 190-195.
[5] Shaltiel-Karyo R, Frenkel-Pinter M, Rockenstein E, et al. A blood-brain barrier (BBB) disrupter is also a potent alpha-synuclein (alpha-syn) aggregation inhibitor: a novel dual mechanism of mannitol for the treatment of Parkinson disease (PD) [J]. J Biol Chem, 2013, 288 (24): 17579-17588.
[6] Schulz-Schaeffer W J. The synaptic pathology of α-synuclein aggregation in dementia with Lewy bodies, Parkinson's disease and Parkinson's disease dementia [J]. Acta Neuropathol, 2010, 120 (2): 131-143.
[7] Specht C G, Tigaret C M, Rast G F, et al. Subcellular localisation of recombinant alpha-and gamma-synuclein [J]. Mol Cell Neurosci, 2005, 28 (2):326-334.
[8] Al-Wandi A, Ninkina N, Millership S, et al. Absence of alpha-synuclein affects dopamine metabolism and synaptic markers in the striatum of aging mice[J]. Neurobiol Aging, 2010, 31 (5):796-804.
[9] Kirkitadze M D, Bitan G, Teplow D B. Paradigm shifts in Alzheimer's disease and other neurodegenerative disorders: the emerging role of oligomeric assemblies [J]. J Neurosci Res, 2002, 69 (5): 567-577.
[10] Yu S, Li X, Liu G, et al. Extensive nuclear localization of alpha-synuclein in normal rat brain neurons revealed by a novel monoclonal antibody [J]. Neuroscience, 2007, 145 (2): 539-555.
[11] 尹娜, 陈予东,李昕,等. 帕金森病患者血浆增强α-突触核蛋白寡聚体形成[J]. 首都医科大学学报. 2013(06) :840-843.
[12] Liu G, Chen M, Mi N, et al. Increased oligomerization and phosphorylation of α-synuclein are associated with decreased activity of glucocerebrosidase and protein phosphatase 2A in aging monkey brains [J]. Neurobiol Aging, 2015, 36 (9): 2649-2659.
[13] 杨斌,杨巍巍,李昕,等. α-突触核蛋白和硝基化α-突触核蛋白在不同年龄段食蟹猴结肠中的表达 [J]. 首都医科大学学报,2014,35(2): 214-218.
[14] 李昕, 杨巍巍,李旭冉,等. 寡聚化α-突触核蛋白在不同年龄段食蟹猴消化道中的表达[J]. 首都医科大学学报,2014,35(5) :592-595.
[15] 张明华, 杨巍巍,李昕,等. 缺血性卒中患者血浆磷酸化α-突触核蛋白水平及磷酸化形成比例的改变[J]. 中国脑血管病杂志,2014,11(9) :476-479.
[16] Li W, Lesuisse C, Xu Y, et al. Stabilization of α-synuclein protein with aging and familial Parkinson's disease-linked A53T mutation [J]. J Neurosci, 2004, 24 (33): 7400-7409.
[17] Chu Y, Kordower J H. Age-associated increases of α-synuclein in monkeys and humans are associated with nigrostriatal dopamine depletion: is this the target for Parkinson's disease [J]. Neurobiology Dis, 2007, 25 (1): 134-149.
[18] Xuan Q, Xu S L, Lu D H, et al. Increase expression of α-synuclein in aged human brain associated with neuromelanin accumulation [J]. J Neural Transm, 2011, 118 (11): 1575-1583.

Differential expressions of oligomer α-synuclein and phosphorylated α-synuclein in synaptosome in the brain regions of different ages of cynomolgus monkeys of different ages

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