Abstract: Objective To establish a method for the detection of pueratin and salvianolic acid B in Guanmaining Tablets, and to study the fingerprint chromatogram of the preparation. Methods HPLC-DAD was used for the quantitative determination of puerarin and salvianolic acid B, and fingerprint chromatogram study of Guanmaining Tablets. The column of Phenomenex Luna C18(250 mm×4.6 mm, 5 μm) was used as a stationary phase, the mixture of methanol and 0.5% formic acid was used as a mobile phase in gradient elution mode, and the detection wavelengths were 250 nm for puerarin and 286 nm for salvianolic acid B. Results Puerarin and salvianolic acid B showed good linear relationships in the ranges of 0.002 523~0.504 6 mg/mL and 0.002 657~0.425 2 mg/mL, respectively. The average recoveries were 99.53%(n=6, RSD=1.5%) for puerarin and 102.2%(n=6, RSD=1.4%) for salvianolic acid B. In the fingerprint study, the similarity computation results of 56 samples ranged from 0.25 to 0.98. Conclusion The method is simple, accurate, and quick and can be used for the quality control in Guanmaining tablets.

Key words: Guanmaining tablets, puerarin, salvianolic acid B, fingerprint chromatogram