Topographic images analysis of mitochondria from α-synuclein transgenic mice utilizing atomic force microscopy

doi:10.3969/j.issn.1006-7795.2016.06.017

Abstract

Abstract: Objective To investigate the mitochondrial topographic images and nanostructural changes in isolated cortex mitochondria from mice overexpressing human wild-type α-synuclein (Thy1-α-synuclein mice, Tg/α-syn) via atomic force microscopy (AFM). Methods Via JC-1 staining to exam the mitochondrial membrane potential of mitochondria isolated from wild type mice(WT) and transgenic mice(TG). The topographic images and nanostructural changes of mitochondria were detected by AFM between the two groups. Results The mitochondrial membrane potential of mitochondria isolated from transgenic mice was decreased by 20.4% compared with the wild type mice (P<0.01). And the topographic images and nanostructure results showed that the length, roughness and number of pore-like structure were increased in mitochondria isolated from TG group (P<0.05). Conclusion Overexpressing α-syn could attenuate mitochondrial membrane potential (Δψ_m) and the mitochondrial membrane was destroyed. The AFM could show the topographic images and nanostructural changes in the isolated mitochondria using tapping mode.

Key words: atomic force microscopy (AFM), mitochondria, α-synuclein (α-syn), mitochondrial membrane potential(Δψ_m)

CLC Number:

R742.5

Gao Ge, Wang Zhipeng, Duan Chunli, Lu Lingling, Yang Hui. Topographic images analysis of mitochondria from α-synuclein transgenic mice utilizing atomic force microscopy[J]. Journal of Capital Medical University, 2016, 37(6): 806-811.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2016.06.017

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2016/V37/I6/806

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