Effect of spatholobus suberectus column extract on apoptosis and mitochondrial transmembrane potential of non-small cell lung cancer A549 in vitro

doi:10.3969/j.issn.1006-7795.2011.06.021

Journal of Capital Medical University ›› 2011, Vol. 32 ›› Issue (6): 811-815.doi: 10.3969/j.issn.1006-7795.2011.06.021

• 基础研究 • Previous Articles Next Articles

Effect of spatholobus suberectus column extract on apoptosis and mitochondrial transmembrane potential of non-small cell lung cancer A549 in vitro

WANG Yan¹, LI Ping¹, LIN Yan¹, ZHAO Jing-xia¹, LIU Xin¹, HE Wei¹, LIANG Dai-ying¹, LUO Xiao-qin², WANG Xiao-min²

1. Beijing Institute of Traditional Chinese Medicine, Beijing 100010, China;2. Beijing Hospital of Traditional Chinese Medicine, Capital Medical University, Beijing 100010, China

Received:2011-03-10 Revised:1900-01-01 Online:2011-12-21 Published:2011-12-21

Abstract

Abstract: Objective To investigate the effect of spatholobus suberectus column extract(SSCE) on apoptosis and mitochondrial transmembrane potential of non-small cell lung cancer A549 in vitro. Methods Cell counting kit-8 was used to investigate the effect of SSCE on the proliferation of A549, apoptotic morphological changes were observed by Annexin V-PI double-staining technique, nuclear changes were observed by Hochest33342 staining, mitochondrial changes were observed by Mitotracker red staining, and mitochondrial membrane potential was examined by JC-1 staining. Results The concentration of 40 mg/L, SSCE could suppress the proliferation of A549 cells in a dose and time-dependent manner. After cultured with SSCE for 24 h, A549 cells exhibited typical characteristics of apoptosis, such as phosphatidylserine(PS) externalization, pyknosis, dense-stained nucleus and cytoplasm, nucleus granulation, mitochondrias gathered to the nucleus and their shape changed from rod into granular form, mitochondrial membrane potential decreased. Conclusion SSCE induced apoptosis of A549 cells by changing the mitochondrial morphology and reducing the mitochondrial membrane potential.

Key words: spatholobus suberectus, A549, apoptosis, mitochondria

CLC Number:

R 284.3

WANG Yan;LI Ping;LIN Yan;ZHAO Jing-xia;LIU Xin;HE Wei;LIANG Dai-ying;LUO Xiao-qin;WANG Xiao-min. Effect of spatholobus suberectus column extract on apoptosis and mitochondrial transmembrane potential of non-small cell lung cancer A549 in vitro[J]. Journal of Capital Medical University, 2011, 32(6): 811-815.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2011.06.021

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2011/V32/I6/811

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Effect of spatholobus suberectus column extract on apoptosis and mitochondrial transmembrane potential of non-small cell lung cancer A549 in vitro

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