Abstract: Objective To study the effects of Gubentongluo decoction on Tubulointerstitial p38MAPK expression in diabetic nephropathy rats. Methods The model of diabetic nephropathy rats was established by unnephrectomized and intraperitoneal injection of streptozotocin(STZ). The rats were divided into six groups randomly, including normal group, model group, western medicine group, Gubentongluo decoction (high, middle, low dosage) groups. Then, the levels of blood glucose and renal function(including serum creatinine, urea nitrogen) were measured in each group of rats. HE staining was applied to observe changes in tubules. Immunohistochemistry was used to observe tubulointerstitial p38MAPK expression. Results Compared with the model group, intragastric administration of Gubentongluo decoction significantly lowered the blood glucose, serum creatinine, urea nitrogen, improved the tubular pathological changes and reduced tubulointerstitial p38MAPK expression. Conclusion Gubentongluo decoction could significantly reduce tubulointerstitial p38MAPK expression, delay the process of renal pathology in DN rats, and has a protective effect on kidney.

Key words: Gubentongluo decoction, diabetic nephropathy, p38MAPK, experimental study