Influence of Qiwei granules on the expression of CD2AP in podocyte of rats with diabetic nephropathy

doi:10.3969/j.issn.1006-7795.2015.02.014

Abstract

Abstract: Objective To investigate the effects of Qiwei granules on the expression of CD2-associated protein(CD2AP) in podocytes of rats with diabetic nephropathy(DN). Methods Ten normal rats were chosen randomly as the normal group, and the others were abdominally administrated with a single injection of streptozotocin(60 mg/kg) to induce diabetic nephropathy rat models.The model rats were separated into 3 groups randomly: the model group, the western medicine group and Qiwei granules group, and successive administration for 24 weeks. Fasting blood glucose(FBG), body weight(BW), 24 h urinary albumin excretion(UAER) were examined every 4 weeks.After 24 weeks, all the rats were sacrificed,and CD2AP in podocyte was detected by immunohistochemistry and Western blotting. Results As compared with normal control group, the BW in model group are significantly decreased(P>0.05), meanwhile the FBG and 24 hUAER are significantly increased(P<0.05). Compared with model group, BW and FBG in administation group did not change(P>0.05), 24 h UAER is decreased(P<0.05), and the expression of CD2AP is significantly increased(P<0.05). Conclusion 24 h UAER is decreased in Qiwei granules group.The protective effects of Qiwei granules group on kidney are achieved by up-regulating the expression of CD2AP.

Key words: Qiwei granules, diabetic nephropathy, podocyte, CD2AP

CLC Number:

R781.6

Wang Xinyao, Geng Jianguo, Gao Yanbin, Li Jiaoyang, Zhou Shengnan, Zou Dawei, Liu Yingxin. Influence of Qiwei granules on the expression of CD2AP in podocyte of rats with diabetic nephropathy[J]. Journal of Capital Medical University, 2015, 36(2): 239-243.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.02.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I2/239

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