Effects of chrysophanol on the expressions of cyclooxygenase-2 and matrix metalloproteinase-9 in the penumbra of mice following focal cerebral ischemia/reperfusion

doi:10.3969/j.issn.1006-7795.2017.01.010

Abstract

Abstract: Objective To explore the effects of Chrysophanol (CHR) on the expression levels of cyclooxygenase-2 (COX2) and matrix metalloproteinase-9 (MMP-9) in mice with middle cerebral artery occlusion (MCAO)/reperfusion injury and to investigate the potential anti-inflammatory mechanism of CHR. Methods Eighteen male C57BL mice were divided into 3 groups randomly:Sham group (n=6), MCAO group (n=6), and CHR group[mice received 0.1 mg/kg CHR (i.p.) for 14 days after ischemia/reperfusion]. MCAO was induced by using the suture method. The mice underwent 45 min of right MCAO, and then reperfusion by withdrawing filament. Rectal temperature was monitored and kept in normal range during the operation. The mice were sacrificed and the brains were harvested on 14 d after reperfusion. The expressions of COX2 and MMP-9 were detected by immunofluorescent staining. And the cellular location of COX2 and MMP-9 were detected by double immunofluorescence labeled antibody technique. Results 1) In Sham group, little COX2 positive cells and MMP-9 positive cells were observed. Compared with Sham group, the number of COX2 positive cells and MMP-9 positive cells increased significantly in the penumbra of MCAO group (P<0.05). 2) Compared with MCAO group, the number of COX2 positive cells and MMP-9 positive cells decreased significantly in the penumbra of MCAO+CHR group on day 14 after reperfusion (P<0.05). 3) COX2-positive immunoreactive cells were colocalized with the general neuronal marker, NeuN, in the penumbra of ischemia/reperfusion mice. And MMP-9-positive immunoreactive cells were colocalized with NeuN. Conclusion CHR has long-term neuroprotective effect against ischemia/reperfusion injury which might be attributed to its anti-inflammatory actions by decreasing the expression of COX2 and MMP-9.

Key words: cerebral ischemia, Chrysophanol, inflammatory middle cerebral artery occlusion, cyclooxygenase-2, matrix metalloproteinase-9

CLC Number:

R743.3

Fang Yalan, Huang Yuyou, Zhao Yongmei, Li Jincheng, Duan Yunxia, Zhao Haiping, Gao Li, Luo Yumin. Effects of chrysophanol on the expressions of cyclooxygenase-2 and matrix metalloproteinase-9 in the penumbra of mice following focal cerebral ischemia/reperfusion[J]. Journal of Capital Medical University, 2017, 38(1): 47-52.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2017.01.010

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2017/V38/I1/47

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