Moderate regulatory effect of normobaric hyperoxia on nuclear factor-κB in cerebral ischemia-reperfusion injured rats

doi:10.3969/j.issn.1006-7795.2018.03.008

Abstract

Abstract: Objective To investigate the effect of normobaric hyperoxia (NBO) on expression of interleukin-1 β (IL-1β), tumor necrosis factor-α (TNF-α), nuclear factor-κB (NF-κB) and inhibitor of NF-κB (IκB) induced by cerebral ischemia-reperfusion injury in rats, and explore the effect of NBO treatment on inflammatory injury mediated by NF-κB after cerebral ischemia.Methods A total of fifteen healthy adult male SD rats (280-320 g) were randomly divided into Sham group (n=3), normoxia group (n=6) or NBO group (n=6). A model of middle cerebral artery occlusion for 90 min and reperfusion for 24 h was induced by using the intraluminal suture method. The sham group and the normoxia group rats breathed normal air, and NBO group rats were exposed to 100% oxygen after ischemia until reperfusion. Immunofluorescence staining and Western blotting were used to observe the expression of IL-1β, TNF-α, NF-κB and IκB in the core cerebral ischemic region.Results Immunofluorescence results showed that:1compared with Sham group, the number of IL-1β, TNF-α or nuclear NF-κB p65 positive cells in the core ischemic region of Normoxia group was increased significantly (P<0.05) with the fluorescence intensity increased; 2compared with Normoxia group, the number of IL-1β, TNF-α or nuclear NF-κB p65 positive cells in the core ischemic region of NBO group decreased significantly (P <0.05) with the fluorescence intensity decreased. Western blotting results showed that:1 compared with Sham group, the expression of NF-κB p65 increased and the expression of IκB decreased significantly in core ischemic region of Normoxia group (P <0.05); 2compared with Normoxia group, the expression of NF-κB p65 decreased and the expression of IκB increased significantly of NBO group (P <0.05).Conclusion NBO treatment may inhibit cerebral ischemia-reperfusion injury by regulating the activation of transcription factor NF-κB in core ischemic region, which imposes an effect on neuroprotection.

Key words: cerebral ischemia, normobaric hyperoxia, inflammation, nuclear factor-κB

CLC Number:

R743

Shi Wenjuan, Zhao Yongmei, Qi Zhifeng, Huang Yuyou, Fang Yalan, Liu Kejian. Moderate regulatory effect of normobaric hyperoxia on nuclear factor-κB in cerebral ischemia-reperfusion injured rats[J]. Journal of Capital Medical University, 2018, 39(3): 349-354.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.03.008

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I3/349

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