Effects of propylthiouracil addition in high fat diet on blood lipid, body weight and body fat of rats

doi:10.3969/j.issn.1006-7795.2018.03.014

Abstract

Abstract: Objective To investigate the effects of propylthiouracil on the body weight, body fat content and adipocyte size in hyperlipidemic rats induced by high-fat diets, and to investigate the applicability of the hyperlipidemia animal model induced by high fat diet containing propylthiouracil.Methods The SD rats were divided into 4 groups:normal diet, normal diet+propylthiouracil, high fat diet and high fat diet+propylthiouracil group, and were given the corresponding feed, and were given propylthiouracil 50 mg·kg^-1·d^-1 by gavage. The animals' feed intake, body weight, blood lipid, thyroid hormone levels were measured, and the Lee's index, liver index, perirenal adipose index, and epididymal adipose index were calculated. Oil red staining of liver tissue and HE staining of epididymal fat were made.Results Addition of propylthiouracil significantly increased lipid levels in animals given high-fat diet, with faster modeling speed and higher serum cholesterol level and low density lipoprotein-cholesterol (LDL-C), and liver lipid deposition.However, with prolonged intake of propylthiouracil, animal feed intake rapidly decreased,followed by animal body weight, perirenal fat and epididymal fat weight, adipocyte area, and Lee's index. At the same time, the animals showed dry and yellow hair, activity reduction, lags in response.Conclusion The addition of propylthiouracil is an effective method of making a model of hypercholesterolemia but with a significant reduction in body weight, body fat, and size of adipocyte.These characteristics were not consistent with the characteristics of common hyperlipidemia associated with high-fat diet in clinical practice. So addition of propylthiouracil is an effective method to mimic hypercholesterolemia associated with hypothyroidism, but it is not appropriate to characterize other types of hyperlipidemia.On the other hand, propylthiouracil has impact on a variety of lipid metabolism enzymes, may interfere with the correct judgment of drug's targets when doing a study on drug's lipid-lowering mechanism, therefore, it should be carefully selected according to the research purposes.

Key words: propylthiouracil (PTU), high fat diet, hyperlipidemia, body weight, body fat, Lee's index

CLC Number:

R965

Hao Weijia, Yang Qiushi, Li Jingyi, Ma Yi, Lu Li, Xiong Jie, Li Yuhang, Xu Pingxiang, Chen Yi, Xue Ming, Li Xiaorong. Effects of propylthiouracil addition in high fat diet on blood lipid, body weight and body fat of rats[J]. Journal of Capital Medical University, 2018, 39(3): 385-392.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2018.03.014

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2018/V39/I3/385

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