Construction of B7-H3 knockdown ES-2 cell line and its effect on cell proliferation

doi:10.3969/j.issn.1006-7795.2021.01.011

Abstract

Abstract: Objective To construct a B7-H3 knockdown ES-2 cell line and determine the effect of B7-H3 gene on the proliferation of ES-2 cell line. Methods Lentivirus mediated shRNA interference was used to establish ES-2 cell line with B7-H3 knockdown. Fluorescence microscopy,real-time fluorescent quantitative PCR(qRT-PCR) and flow cytometry were used to verify the effect of B7-H3 knockdown, and cell counting kit-8 (CCK-8) was used to test its effect on proliferation. Results The results of CCK-8 showed that the proliferation rate of the experimental group was significantly lower than that of the control group, with statistical significance(P<0.05). Conclusion B7-H3 knockdown ES-2 cell line is successfully constructed, and it is found that B7-H3 knockdown can significantly reduce the proliferation rate of ES-2 cells.

Key words: ES-2 cell line, B7-H3, shRNA interference technology, cell proliferation

CLC Number:

R713.6

Deng Mengqi, Zhang Yanqin, Chang Xiangyu, Wu Di, Xu Chunyu, Miao Jinwei. Construction of B7-H3 knockdown ES-2 cell line and its effect on cell proliferation[J]. Journal of Capital Medical University, 2021, 42(1): 66-70.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2021.01.011

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2021/V42/I1/66

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