Journal of Capital Medical University ›› 2006, Vol. 27 ›› Issue (2): 143-146.

• 专题报道 • Previous Articles     Next Articles

High Frequency CTL Responses in HIV-1-Infected Chinese Plasma Donors

Zhao Chunhui1, Xu Keyi2, Chen Xinyue1, Wu Hao1, Yan Huiping1, Zhang Yonghong1, Lun Wenhui2, Xu Xiaoning3, Andrew McMichael3, Sarah Rowland-Jones3, Dong Tao3   

  1. 1. Beijing You'an Hospital, Capital University of Medical Sciences;2. Beijing Ditan Hospital;3. Institute of Molecule Medicine University of Oxford
  • Received:2006-02-28 Revised:1900-01-01 Online:2006-04-24 Published:2006-04-24

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Abstract: Objective To study the magnitude and breadth of HIV specific T cell responses in 8 HIV infected long-term nonprogressors(LTNPs) and slow progressors(SP) who received contaminated blood.Methods PBMCs were tested in an Elispot assay against a matrix of peptides(15 mers overlapping by 11 amino acids) representing the full proteome of HIV clade B virus.The peptides were presented as 3 dimensional matrixes such that each peptide appears in 3 unique wells enabling the epitope to be identified.Results We found broad T cell responses in most donors,although in one individual,a strong response against only gag protein was observed and T cells responses to pol,gag and nef were more dominate than the others.Conclusion High frequency and broad HIV specific T cells detected in Chinese HIV infected LTNP and SP individuals are very likely associated with the control of the disease progression in those individuals.

Key words: human immunodeficiency virus(HIV), acquired immunodeficiency syndrome(AIDS), HIV-specific cytotoxic T lymphocytes, HIV-1 long-term nonprogressors(LTNPs)

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