Effects of Pioglitazone on neuronal damage in the mouse hippocampus after middle cerebral artery occlusion and reperfusion

doi:10.3969/j.issn.1006-7795.2023.04.022

Abstract

Abstract: Objective To investigate the potential neuroprotective effects and the underlying mechanisms of Pioglitazone(PGZ) on neuronal damage in mice after cerebral ischemia. Methods A total of 36 young-aged (2-month) C57 BL/6J mice were randomly divided into 3 group (n=12): sham group, vehicle group, and PGZ group. Mice were treated with PGZ (25 mg/kg) or saline for 7 d by intragastric administration after transient middle cerebral artery occlusion (MCAO). Neurological function was evaluated by Neurological Severity Sores (NSS) at 3, and 7 d after MCAO.Infarction volume and the number of neurons in cortex, striatum, and the hippocampal CA1, CA3, dentate gyms (DG) regions were measured by Nissl staining.Western blotting was used to assess the expression of apoptosis associated protein B-cell leukemia/lymphoma 2 (Bcl2) and Bcl2 associated X protein(Bax) at 7 d after MCAO. Results Compared to the vehicle-treated mice, PGZ-treated mice didn't exhibit better performance at 3, 7 d after MCAO surgery (P>0.05). However, PGZ reduced infarct volume of the brains, and PGZ also significantly ameliorated neuron loss in cortex and hippocampal CA3 region after cerebral injury compared with the vehicle group (P<0.05). Moreover, PGZ promoted the ratio of Bcl2 and Bax (P<0.05).Conclusions These results suggest that PGZ treatment alleviated the neuronal damage in hippocampal CA3region of mice with cerebral ischemia-reperfusion injury, which may be explained by PGZ regulated the ratio of Bcl2 and Baxin hippocampus.

Key words: Pioglitazone, cerebral ischemia, neuronal damage, neuroprotection

CLC Number:

Wang Rongliang, Yan Feng, Tian yue, Huang Yuyou, Luo Yumin, Ma Shubei. Effects of Pioglitazone on neuronal damage in the mouse hippocampus after middle cerebral artery occlusion and reperfusion[J]. Journal of Capital Medical University, 2023, 44(4): 645-651.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2023.04.022

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2023/V44/I4/645

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