Abstract:

Objective To investigate the effects of angiotensinⅡ(AngⅡ) and angiotensinⅡ type 1 receptor antagonist (AT1RA) on the collagenⅠsynthesis and collagenⅠmRNA expression of cultured hepatic stellate cells (HSCs) in vitro. Methods HSC-T6 rat hepatic stellate cell line was chosen as the study model of the activated hepatic stellate cells. Cultured HSCs were randomized into control group, AngⅡgroup, AT1RA group and AngⅡ+AT1RA group. Cell culture medium was used to detect the collagenⅠlevel with ELISA method. HSCs were harvested to measure the collagenⅠmRNA expression with RT-PCR. Results The collagen Ⅰ level and collagen Ⅰ mRNA expression level of AngⅡ group was higher than control group(P<0.05), AT1RA group and AngⅡ+AT1RA group was lower than control group(P<0.05). Conclusion AngiotensinⅡ can increase the collagenⅠ synthesis and collagenⅠ mRNA expression of hepatic stellate cells, while all these effects are inhibited by angiotensinⅡ type 1 receptor antagonist.

Key words: angiotensinⅡ, angiotensinⅡ receptor antagonist, hepatic stellate cell, collagen