Abstract: Objective To study the therapeutic effects of artificial liver support system treatment for in acute liver failure.Methods Eleven miniature pigs were divided into the therapeutic and control groups.Pigs were given D-galactosamine to build models of acute liver failure.Pigs in the therapeutic group were treated after 48 h with plasma exchange and hemofiltration of the artificial liver support system(ALSS).Four parameters were observed: survival time,common condition,biochemical assay,changes in liver pathology.Results The survival time(128.7±11.3) h in the therapeutic group were remarkably different from those in the control group(67.9±9.4)h in terms of improvements in the common condition,as well as ALT,TBil and PT levels(P<0.01).Area of necrosis was obviously reduced in the therapeutic group,and cholestasis was not found.Conclusion Treatment of acute liver failure by ALSS in pig model is very effective.

Key words: ALSS, ALF, PCNA, biochemical assay, changes in hepatic pathology