A Murine Model of Cryptococcus Neoformans Infection and PCR Rapid Diagnosis

doi:10.3969/j.issn.1006-7795.2009.05.023

Abstract

Abstract: Objective The authors established a cryptococcus neoformans-inoculated murine model which can be used to explore the diagnostic effect of cryptococcosis by PCR method. Methods The murine model for PCR diagnostic use was made by inoculating cryptococcus agents through tracheal intubation in BALB/c mice. PCR diagnosis was tested on DNA samples extracted from lung tissue of challenged mice. Results A pair of primers(ITS1-CN4) from eight pairs of candidate primers were screened out, which targeted the ribosomal DNA gene cluster and ITS1, ITS2 regions and could detect cryptococcus from murine tissue sensitively and specifically. Conclusion The murine model of cryptococcus neoformans infection were successfully established. One pair of primers(ITS1-CN4) could detect cryptococcus from murine tissue with high sensitivity and specificity.

Key words: cryptococcosis, PCR diagnosis, animal model

CLC Number:

R 519.4

YI En-tong;YIN Cheng-hong;LI Min. A Murine Model of Cryptococcus Neoformans Infection and PCR Rapid Diagnosis[J]. Journal of Capital Medical University, 2009, 30(5): 682-686.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2009.05.023

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2009/V30/I5/682

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A Murine Model of Cryptococcus Neoformans Infection and PCR Rapid Diagnosis

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