The effect of small dose DMBA on rat pancreatic tumorigenesis

doi:10.3969/j.issn.1006-7795.2015.06.018

Abstract

Abstract: Objective To explore the possibility of pancreatic carcinogenesis induced by small dose 7,12-dimethylbenzanthracene(DMBA) implantation in Sprague-Dawley(SD) rats. Methods Totally 70 male SD rats were randomly divided into two groups: DMBA(2 mg/100 g) was implanted into the parenchyma of rats pancreas in experimental group(n=60);No drugs were implanted into the parenchyma of pancreas in control group. Rats were killed after surgery operation(2, 4 and 6 months). Finally, the pancreases of rats were fixed in formalin for HE staining and immunohistochemical analysis. Results 1 No significant differences were observed with the weight of rats between experimental and control groups(P=0.680);2 The incidence of pancreatic cyst was 33.3% in the experimental group 4 months after surgery. The incidence of pancreatic cyst was 38.9% and that of leiomyosarcoma was 5.6% in the experimental group 6 months after surgery. Conclusion Small dose of DMBA(2 mg/100 g) implanted into SD rats can cause acute pancreatitis, chronic pancreatitis, pancreatic cyst, and pancreatic leiomyosarcoma. The incidence of pancreatic leiomyosarcoma is very low. But no pancreatic ductal cell carcinoma was found.Further reaserch is needed.

Key words: pancreatic tumor, sarcoma, 7,12-dimethylbenzanthracene, animal model

CLC Number:

R735.9

Ren Yu, Hou Xiaopu, Li Dan, Li Ying, Zhu Bin. The effect of small dose DMBA on rat pancreatic tumorigenesis[J]. Journal of Capital Medical University, 2015, 36(6): 936-941.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.06.018

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I6/936

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