Detection of vancomycin-resistant enterococcus faecium by MALDI-TOF MS

doi:10.3969/j.issn.1006-7795.2015.03.021

Abstract

Abstract: Objective To evaluate a rapid matrix-assisted laser desorption ionization-time of flight mass spectrometry (MAIDI-TOF MS) assay in identifying vancomycin-resistant Enterococcus faecium. Methods Totally 150 enterococcus faecium clinical strains collected from 2009 to 2012 were studied, including 60 vancomycin-resistant enterococcus faecium (VRE) isolates and 90 vancomycin-susceptible (VSE) strains. Resistance genes were detected by gene sequencing. Spectra of 30 VRE and 30 VSE which were randomly chosen, were analyzed by ClinPro Tools program of MALDI-TOF MS in order to screen the discrepancy mass peaks according to ROC curve. 30 VRE and 60 VSE were detected by MALDI-TOF MS according to the discrepancy mass peaks. Results All strains were verified as enterococcus faecium by MALDI-TOF MS. 60 VRE strains carried vanA gene, and 90 VSE strains carried no vancomycin resistant gene. Two discrepancy mass peaks between the two groups of strains were 3516.14, 3644.12, of which AUC were 0.782, 0.767, respectively. Through the two discrepancy mass peaks, performance of VRE detection method by MALDI-TOF MS were below. Sensitivity=80%, specificity=90%, false positive rate=10%, false negative rate=10%, positive predictive value=80%, negative predictive value=90%. Conclusion MALDI-TOF MS can be used accurately to identify VRE, and guide the clinical rational use of antibiotics.

Key words: enterococcus faecium, vancomycin, mass spectrometry, resistance

CLC Number:

R446.1

Wu Wei, Wang Lijun, Sui Wenjun, Zhang Gui. Detection of vancomycin-resistant enterococcus faecium by MALDI-TOF MS[J]. Journal of Capital Medical University, 2015, 36(3): 444-448.

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URL: https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/10.3969/j.issn.1006-7795.2015.03.021

https://journal03.magtech.org.cn/Jweb_sdykdxxb/EN/Y2015/V36/I3/444

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