Journal of Capital Medical University ›› 2026, Vol. 47 ›› Issue (3): 428-436.doi: 10.3969/j.issn.1006-7795.2026.03.004

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Construction of a Pd/Cu-TCPP-based photoresponsive nanosystem and preliminary investigation of its synergistic antitumor mechanism

Ren Yihan1，2，3，4，5, Jiang Yuyan1，2，3，4，5, Sun Qi1，2，3，4，5*   

  1. 1.Department of Pharmaceutics, School of Pharmaceutical Sciences, Capital Medical University, Beijing 100069, China; 2. Laboratory for Clinical Medicine （Laboratory of Pediatric Tumor Pathogenesis and Innovative Drug Research）, Capital Medical University, Beijing  100069, China；3. Beijing Area Major Laboratory of Peptide and Small Molecular Drugs, Beijing 100069, China， 4. Engineering Research Center of Endogenous Prophylactic of Ministry of Education of China, Beijing 100069,  China; 5.Beijing Laboratory of Biomedical Materials, Beijing 100069, China
  • Received:2026-02-02 Revised:2026-04-09 Online:2026-06-21 Published:2026-06-26
  • Supported by:
    This study was supported by National Natural Science Foundation of China ( 82304389)， Beijing Municipal Education Commission General Research Program (KM202310025023)，Beijing Natural Science Foundation Undergraduate “Qiyan” Research Program (QY25430).

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Abstract: Objective  To construct a Pd/Cu-TCPP (PCT)-based photoresponsive nanodelivery system (PCT@PDA/siPKM2), investigate the feasibility of synergistic photothermal therapy, hydrogen therapy, and gene therapy, and preliminarily evaluate its in vitro antitumor mechanism.Methods  PCT nanoparticles were synthesized via a solvothermal method and subsequently coated with polydopamine (PDA) to form a core-shell structure. Polyethylenimine (PEI) was further employed to load siRNA targeting the PKM2 gene (siPKM2), yielding PCT@PDA/siPKM2 nanoparticles. The physicochemical properties, siRNA loading and protection capacity, photothermal performance, and hydrogen release behavior were systematically characterized. Cellular uptake and antitumor efficacy were evaluated in 4T1 mouse breast cancer cell line.Results  Uniform and stable PCT@PDA/siPKM2 nanoparticles were prepared, exhibiting excellent siRNA loading and protection capabilities, as well as prominent photothermal conversion efficiency and controllable hydrogen release under 808 nm near-infrared laser irradiation. In vitro studies demonstrated effective lysosomal escape and significant inhibition of 4T1 cell viability upon laser irradiation.Conclusion  This study confirmed the feasibility of PCT@PDA/siPKM2 in combining photothermal effects, hydrogen release and genetic intervention, providing an experimental basis for further research into synergistic tumour control.

Key words: metal-porphyrin coordination, polydopamine, pyruvate kinase M2, siRNA, photoresponsive nanomaterials, synergistic antitumor mechanism

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