Abstract:

Attempts were made to cultivate Giardia lamblia monoxenically and axenically and to study its morph-ology and multiplication during cu-ltivation.The observations were made with optic microscope and scanning electromicroscope.Rabb-its were artificially infected with partially excysted cysts isolated from patients.The distribution of the parasite over the mucosa sur-face of the intestine and the path-ologic changes therein of the inf-ected animal were studied.Fecal specimens of 1596 individuals from several districts were examined and giardia incidence among them ave-raged 2.7%(adults 0.9%,Childr-en 5.3%).Sera of 541 of these individuals were also tested simul-taneously with enzyme-linked imm-unosorbent assay (ELISA) conduc-ted with antigen prepared from axenic culture of the organism. The antibody prevelence rate was 12.8%.Counterimmunoelectropho-resis(CIE)was found to be a very useful tool in the diagnosis of giar-diasis.A typical case of familial infection was also reported.