Abstract:

Demineralisation of enamel on sound human premolars was produced by Streptococcus mutans using a sequential culture technique. Adhesion of Streptococcus mutans on emamel surface and formation of plaque were observed by scanning electronic microscope. Initial enamel lesion on sections were detected in microradiography under transmitted and polarized light.This study established a method for the production of caries-like lesion,so that the aetiology of dental caries and the prevention of caries can be studied.